Quantitative trait loci analysis of nitrogen use efficiency in Arabidopsis
Quantitative trait loci analysis of nitrogen use efficiency in Arabidopsis
复制标题
DOI：
10.1104/pp.102.010785
复制
发表时间：
2003-01-01
期刊：
影响因子：
7.4
通讯作者：
Daniel-Vedele, F
中科院分区：
文献类型：
--
作者：
Loudet, O;Chaillou, S;Daniel-Vedele, F
提高植物氮素利用效率或控制土壤氮素需要更好地了解植物氮素代谢的调控。这可以用拟南芥作为模式遗传系统，利用生态型之间的自然变异来实现。在此，我们利用数量性状基因座(QTL)对海湾-0x沙赫达拉重组自交系群体的氮代谢变异进行了广泛的研究。我们定位了地上部生长、总氮、硝酸盐和游离氨基酸含量等性状的QTL，这些性状是在两种不同的氮环境(对比土壤中的硝酸盐有效性)下在受控条件下测量的。所有性状都存在遗传变异和超亲现象，大部分遗传变异是通过QTL和QTL X QTL的上位性互作来鉴定的。这48个显著QTL代表了至少18个亲本间的多态基因，其中一些可能对应于氮素代谢途径的已知基因，但也有一些代表控制或与氮素生理相互作用的新基因。性状之间的相关性通过QTL共定位来剖析：对不同性状起调节作用的个体因素的识别为揭示这些性状之间的关系提供了新的线索。我们还指出，我们的性状的调节主要是特定于N环境(N的有效性)。最后，我们描述了四个有趣的基因座，在它们的位置克隆是可行的。
Improving plant nitrogen (N) use efficiency or controlling soil N requires a better knowledge of the regulation of plant N metabolism. This could be achieved using Arabidopsis as a model genetic system, taking advantage of the natural variation available among ecotypes. Here, we describe an extensive study of N metabolism variation in the Bay-0 x Shahdara recombinant inbred line population, using quantitative trait locus (QTL) mapping. We mapped QTL for traits such as shoot growth, total N, nitrate, and free-amino acid contents, measured in two contrasting N environments (contrasting nitrate availability in the soil), in controlled conditions. Genetic variation and transgression were observed for all traits, and most of the genetic variation was identified through QTL and QTL X QTL epistatic interactions. The 48 significant QTL represent at least 18 loci that are polymorphic between parents; some may correspond to known genes from the N metabolic pathway, but others represent new genes controlling or interacting with N physiology. The correlations between traits are dissected through QTL colocalizations: The identification of the individual factors contributing to the regulation of different traits sheds new light on the relations among these characters. We also point out that the regulation of our traits is mostly specific to the N environment (N availability). Finally, we describe four interesting loci at which positional cloning is feasible.