A combination of Flt3 ligand cDNA and CpG ODN as nasal adjuvant elicits NALT dendritic cells for prolonged mucosal immunity.
A combination of Flt3 ligand cDNA and CpG ODN as nasal adjuvant elicits NALT dendritic cells for prolonged mucosal immunity.
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DOI：
10.1016/j.vaccine.2008.06.091
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发表时间：
2008-09-02
期刊：
影响因子：
5.5
通讯作者：
Fujihashi K
中科院分区：
文献类型：
--
作者：
Fukuiwa T;Sekine S;Kobayashi R;Suzuki H;Kataoka K;Gilbert RS;Kurono Y;Boyaka PN;Krieg AM;McGhee JR;Fujihashi K
目的：探讨Flt 3配体cDNA质粒（pFL）与CpG寡核苷酸（CpG ODN）联合作为鼻佐剂的细胞和分子机制。与OVA一起给予的双DNA佐剂在最后一次免疫后的25周内维持了外部分泌物中的OVA特异性分泌型伊加（S-IgA）Ab应答。此外，Th 1型和Th 2型细胞因子应答均由该组合佐剂方案诱导。给予联合佐剂的小鼠的鼻咽相关淋巴网状组织（NALT）中浆细胞样DC（pDC）和CD 8 + DC的频率显著增加。重要的是，当我们在2岁小鼠中检测pFL加CpG ODN的佐剂活性时，也诱导了显著水平的粘膜伊加Ab应答。这些结果表明，鼻腔递送组合的DNA佐剂为开发用于老年人的有效粘膜疫苗提供了有吸引力的可能性。
We explore cellular and molecular mechanisms of nasal adjuvant of a combination of a plasmid encoding the Flt3 ligand cDNA (pFL) and CpG oligodeoxynucleotides (CpG ODN). The double DNA adjuvant given with OVA maintained prolonged OVA-specific secretory IgA (S-IgA) Ab responses in external secretions for more than twenty-five weeks after the final immunization. Further, both Th1- and Th2-type cytokine responses were induced by this combined adjuvant regimen. The frequencies of plasmacytoid DCs (pDCs) and CD8+ DCs were significantly increased in nasopharyngeal-associated lymphoreticular tissue (NALT) of mice given the combined adjuvant. Importantly, when we examined adjuvanticity of pFL plus CpG ODN in 2-year-old mice, significant levels of mucosal IgA Ab responses were also induced. These results demonstrate that nasal delivery of a combined DNA adjuvant offers an attractive possibility for the development of an effective mucosal vaccine for the elderly.
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DOI：
10.1084/jem.20020045
发表时间：
2002-04-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gilliet M;Boonstra A;Paturel C;Antonenko S;Xu XL;Trinchieri G;O'Garra A;Liu YJ
通讯作者：
Liu YJ
FLT3配体处理的小鼠功能成熟的树突状细胞数量的急剧增加：确定的多个树突状细胞亚群。
影响因子：
15.3
作者：
Maraskovsky, E;Brasel, K;Teepe, M;Roux, ER;Lyman, SD;Shortman, K;McKenna, HJ
通讯作者：
McKenna, HJ
影响因子：
4.4
作者：
Kataoka, K;McGhee, JR;Fujihashi, K
通讯作者：
Fujihashi, K
影响因子：
4.3
作者：
Halpern, MD;Kurlander, RJ;Pisetsky, DS
通讯作者：
Pisetsky, DS
影响因子：
4.4
作者：
Hagiwara, Y;McGhee, JR;Fujihashi, K
通讯作者：
Fujihashi, K