Determination of reference intervals for serum creatinine, creatinine excretion and creatinine clearance with an enzymatic and a modified Jaffe method
Determination of reference intervals for serum creatinine, creatinine excretion and creatinine clearance with an enzymatic and a modified Jaffe method
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DOI：
10.1016/j.cccn.2004.02.007
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发表时间：
2004-06-01
影响因子：
5
通讯作者：
Klein, G
中科院分区：
文献类型：
--
作者：
Junge, W;Wilke, B;Klein, G
目的：本研究的目的是确定参考区间的血清肌酐，肾肌酐输出量和肌酐清除率（CrCl）与两种新的方法，准确测定肌酐。方法：参考人群包括252名健康受试者（男127名，女125名），年龄18 - 74岁，中位数27岁。准确采集尿液24 h。血清和尿液中的肌酸激酶用酶测定法（“Creatinine Plus”）和动力学Jaffe反应的改良法（称为“Jaffe补偿”）测量。结果如下：血清肌酐的参考值几乎与先前发表的用相同方法获得的参考值相同：酶法测定的男性和女性分别为0.73-1.18和0.55-1.02 mg/dl，补偿Jaffe法（Jcomp）测定的分别为0.72-1.16和0.55-0.96 mg/dl。CrCl值呈正态分布，与先前的一些研究相比，没有显示出性别差异。发现整个组的参考区间为66-143 ml/min（酶法）和71-151 ml/min（化学法）。(C)2004 Elsevier B. V.保留所有权利。
Objectives: The aim of this study was to determine the reference intervals for serum creatinine, the renal creatinine output and the creatinine clearance (CrCl) with two new methods for accurate creatinine determination. Methods: The reference population consisted of 252 healthy subjects (127 males and 125 females) at the age between 18 and 74 years, median 27. Urine was collected for exactly 24 h. Creatinine in serum and urine was measured with an enzymatic assay ("Creatinine Plus") and a modification of the kinetic Jaffe reaction, named "Jaffe compensated". Results: Reference values for serum creatinine were almost identical to previously published ones obtained with the same methods: 0.73-1.18 and 0.55-1.02 mg/dl for males and females, respectively, with the enzymatic, 0.72-1.16 and 0.55-0.96 mg/dl with the compensated Jaffe method (Jcomp). CrCl values were normally distributed and showed no gender difference in contrast to some previous studies. The reference interval for the entire group was found to be 66-143 ml/min with the enzymatic assay and 71-151 ml/min with the chemical one. (C) 2004 Elsevier B.V. All rights reserved.