Practical preparation of lacto-N-biose I, a candidate for the bifidus factor in human milk
Practical preparation of lacto-N-biose I, a candidate for the bifidus factor in human milk
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DOI：
10.1271/bbb.70320
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发表时间：
2007-08-01
影响因子：
1.6
通讯作者：
Kitaoka, Motomitsu
中科院分区：
文献类型：
--
作者：
Nishimoto, Mamoru;Kitaoka, Motomitsu
证明了一锅酶促反应以产生乳-N-二糖I（LNB），其被认为代表人乳低聚糖中的双歧因子。在UDP-Glc和磷酸盐存在下，通过蔗糖磷酸化酶、UDP-葡萄糖-己糖-1-磷酸尿苷酰转移酶、UDP葡萄糖4-差向异构酶和乳-N-二糖磷酸化酶这四种酶的同时作用，在10升最初含有660 mM蔗糖和600 mM GlcNAc的反应混合物中产生约500 mM LNB，表明反应产率为83%。在酵母处理后通过结晶从混合物中分离LNB。最后，在重结晶后回收1.4kg纯度为99.6%的LNB。
A one-pot enzymatic reaction to produce lacto-N-biose I (LNB), which is supposed to represent the bifidus factor in human milk oligosaccharides, was demonstrated. Approximately 500 mM of LNB was generated in 10-liter of reaction mixture initially containing 660 m,mM of sucrose and 600 mm of GlcNAc by the concurrent actions of four enzymes, sucrose phosphorylase, UDP-glucose-hexose-1-phospate uridylyltransferase, UDPglucose 4-epimerase, and lacto-N-biose phosphorylase, in the presence of UDP-Glc and phosphate, indicating a reaction yield of 83%. LNB was isolated from the mixture by crystallization after yeast treatment. Finally, 1.4 kg of LNB of 99.6% purity was recovered after recrystallization.