Simultaneous separation of anionic, cationic, and neutral components in capillary liquid chromatography using mixed-bed column of hydrophilic and anion-exchange stationary phases.
Simultaneous separation of anionic, cationic, and neutral components in capillary liquid chromatography using mixed-bed column of hydrophilic and anion-exchange stationary phases.
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DOI：
10.1002/jssc.200800509
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发表时间：
2009-02
影响因子：
3.1
通讯作者：
Naoki Igawa;S. Kitagawa;H. Ohtani
中科院分区：
文献类型：
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作者：
Naoki Igawa;S. Kitagawa;H. Ohtani
同时分离的阴离子，阳离子和中性化合物进行了在毛细管LC使用混合床柱的两性离子亲水性和阴离子交换固定相。固定相中的组合适用于保留阴离子、阳离子和中性分析物。随着移动的相中缓冲液浓度的增加，阴离子化合物的保留受到抑制，而阳离子和中性化合物的保留几乎不变，即，阴离子分析物主要通过阴离子交换方式保留，其它化合物主要通过亲水作用保留。亲水性和静电保留均成功地由移动的相中的ACN含量控制。在移动的相中增加水含量的梯度洗脱在6 min内实现了代谢组学分析中发现的8种化合物的良好分离。每8种化合物的洗脱时间的日内（n = 7）和日间（n = 3）精密度（%RSD）分别小于3%和5%。
A simultaneous separation of anionic, cationic, and neutral compounds was carried out in capillary LC using a mixed-bed column of zwitterionic hydrophilic and anion-exchange stationary phases. The combination in stationary phases was suitable to retain anionic, cationic, and neutral analytes. The retentions of anionic compounds were suppressed with the increase in the buffer concentration in the mobile phase whereas those for the cationic and neutral compounds were almost constant, i.e., the anionic analytes were mainly retained by anion-exchange mode and the other compounds by the hydrophilic interaction. Both hydrophilic and electrostatic retentions were successfully controlled by the ACN content in the mobile phase. The gradient elution of increasing water content in the mobile phase achieved good separation for eight compounds found in metabolome analysis within 6 min. Both intra- (n = 7) and inter-day (n = 3) precisions (%RSD) of elution time for every eight compounds were less than 3 and 5%, respectively.