Interleukin-17F increases the secretion of interleukin-8 and the expression of cyclooxygenase 2 in endometriosis
Interleukin-17F increases the secretion of interleukin-8 and the expression of cyclooxygenase 2 in endometriosis
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DOI：
10.1016/j.fertnstert.2011.04.060
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发表时间：
2011-07-01
影响因子：
6.7
通讯作者：
Taketani, Yuji
中科院分区：
文献类型：
--
作者：
Hirata, Tetsuya;Osuga, Yutaka;Taketani, Yuji
目的：探讨白细胞介素(IL)-17F对子宫内膜异位症间质细胞IL-8分泌及环氧化酶2 （COX2）基因表达的影响。设计：使用人体样本进行体外实验研究。单位：大学医院。患者：子宫内膜异位症组织来自于接受腹腔镜手术的卵巢子宫内膜异位症患者。干预：用IL-17F培养子宫内膜异位症基质细胞（ESCs）。主要观察指标：采用特异性ELISA检测IL-8浓度，实时逆转录聚合酶链反应（PCR）检测IL-8和COX2信使RNA水平。结果：IL-17F增加内皮细胞中IL-8的分泌，且该作用被IL-17受体A和IL-17受体c抗体抑制。肿瘤坏死因子α (tnf - α)协同增强IL-17F诱导的内皮细胞中IL-8分泌的增加。IL-17F增加了内皮细胞中IL-8和COX2基因的表达。结论：提示IL-17F可能通过上调IL-8和COX2来刺激子宫内膜异位症的发生。[j] .中国农业科学（英文版），2011;26(6):591 - 591。(C)2011年美国生殖医学学会。)
Objective: To examine the effects of interleukin (IL)-17F on the secretion of IL-8 and the gene expression of cyclooxygenase 2 (COX2) in endometriotic stromal cells.Design: In vitro experimental study using human samples.Setting: University hospital.Patient(s): Endometriotic tissues were obtained from women with ovarian endometriomas undergoing laparoscopic surgery.Intervention(s): Endometriotic stromal cells (ESCs) were cultured with IL-17F.Main Outcome Measure(s): Concentrations of IL-8 were measured by a specific ELISA, and messenger RNA levels of IL-8 and COX2 were measured by real-time reverse transcription-polymerase chain reaction (PCR).Result(s): IL-17F increased the secretion of IL-8 from ESCs, and the effect was inhibited by antibodies for IL-17 receptor A and IL-17 receptor C. Tumor necrosis factor alpha (TNF-alpha) synergistically enhanced IL-17F-induced increase in IL-8 secretion from ESCs. The IL-17F increased the gene expression of IL-8 and COX2 in ESCs.Conclusion(s): These findings suggest that IL-17F may stimulate the development of endometriosis by up-regulation of IL-8 and COX2. (Fertil Steril (R) 2011;96:113-7. (C)2011 by American Society for Reproductive Medicine.)