Trunk neural crest origin of dermal denticles in a cartilaginous fish
Trunk neural crest origin of dermal denticles in a cartilaginous fish
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DOI：
10.1073/pnas.1713827114
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发表时间：
2017-12-12
影响因子：
11.1
通讯作者：
Criswell, Katharine E.
中科院分区：
文献类型：
--
作者：
Gillis, J. Andrew;Alsema, Els C.;Criswell, Katharine E.
软骨鱼类（例如，鲨鱼和冰鞋）具有由嵌入其皮肤内的齿状“牙齿”组成的颅后皮肤骨骼。与牙齿一样，真皮层的主要骨骼组织是牙本质。在头部，颅神经嵴细胞产生牙齿的牙本质生成细胞（成牙本质细胞）。然而，躯干神经嵴细胞通常被认为是非成骨的，因此躯干齿状突成牙本质细胞的胚胎起源仍然没有得到解决。在这里，我们使用FoxD3的表达，以查明规格和迁移的干神经嵴细胞的软骨鱼，小滑冰（银鱼）的胚胎。使用细胞谱系追踪，我们进一步证明，躯干神经嵴细胞，事实上，引起成牙本质细胞的躯干真皮dendritis。这些研究结果扩大了正常发育过程中脊椎动物躯干神经嵴细胞命运的库，突出了该细胞群可能的原始成骨潜力，并指出整个祖先脊椎动物真皮骨骼的牙本质神经嵴起源。
Cartilaginous fishes (e.g., sharks and skates) possess a postcranial dermal skeleton consisting of tooth-like "denticles" embedded within their skin. As with teeth, the principal skeletal tissue of dermal denticles is dentine. In the head, cranial neural crest cells give rise to the dentine-producing cells (odontoblasts) of teeth. However, trunk neural crest cells are generally regarded as nonskeletogenic, and so the embryonic origin of trunk denticle odontoblasts remains unresolved. Here, we use expression of FoxD3 to pinpoint the specification and emigration of trunk neural crest cells in embryos of a cartilaginous fish, the little skate (Leucoraja erinacea). Using cell lineage tracing, we further demonstrate that trunk neural crest cells do, in fact, give rise to odontoblasts of trunk dermal denticles. These findings expand the repertoire of vertebrate trunk neural crest cell fates during normal development, highlight the likely primitive skeletogenic potential of this cell population, and point to a neural crest origin of dentine throughout the ancestral vertebrate dermal skeleton.