Engineering Butanol-Tolerance in Escherichia coli With Artificial Transcription Factor Libraries
Engineering Butanol-Tolerance in Escherichia coli With Artificial Transcription Factor Libraries
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DOI：
10.1002/bit.22989
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发表时间：
2011-04-01
影响因子：
3.8
通讯作者：
Kim, Sun Chang
中科院分区：
文献类型：
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作者：
Lee, Ju Young;Yang, Kyung Seok;Kim, Sun Chang
大肠杆菌具有生长速度快、易于遗传操作等优点，已被探索作为丁醇生产的宿主菌。丁醇毒性是利用大肠杆菌生产生物丁醇的主要问题。杆菌特别是E.大肠杆菌的生长受到丁醇的严重抑制，1%（vol/vol）丁醇几乎完全停止。本研究开发了一种提高E.用人工转录因子（ATF）文库构建了锌指（ZF）DNA结合蛋白和大肠杆菌（E. coli环腺苷酸受体蛋白（CRP）。利用这些ATFs，我们选择了一个丁醇耐受的E。大肠杆菌可以耐受高达1.5%（vol/vol）的丁醇，同时耐热性增加。我们还鉴定了E.与丁醇耐受性相关的大肠杆菌。结果表明，E.大肠杆菌可以被改造为高产丁醇的有希望的宿主。Biotechnol. Bioeng. 2011; 108：742-749。(C)2010 Wiley Periodicals，Inc.
Escherichia coli has been explored as a host for butanol production because of its many advantages such as a fast growth and easy genetic manipulation. Butanol toxicity, however, is a major concern in the biobutanol production with E. coli. In particular, E. coli growth is severely inhibited by butanol, being almost completely stopped by 1% (vol/vol) butanol. Here we developed a new method to increase the butanol-tolerance of E. coli with artificial transcription factor (ATF) libraries which consist of zinc finger (ZF) DNA-binding proteins and an E. coli cyclic AMP receptor protein (CRP). Using these ATFs, we selected a butanol-tolerant E. coli which can tolerate up to 1.5% (vol/vol) butanol, with a concomitant increase in heat resistance. We also identified genes of E. coli that are associated with the butanol-tolerance. These results show that E. coli can be engineered as a promising host for high-yield butanol production. Biotechnol. Bioeng. 2011; 108: 742-749. (C) 2010 Wiley Periodicals, Inc.