High efficiency gene delivery into laryngeal muscle with bidirectional electroporation.
High efficiency gene delivery into laryngeal muscle with bidirectional electroporation.
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通过双向电穿孔将基因高效递送至喉肌。
DOI：
10.1016/j.otohns.2006.04.003
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Flint,PaulW
中科院分区：
文献类型：
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作者：
Saito,Koichiro;Lehar,Mohamed;Li,ZhaoBo;Braga,Natasha;King,AlanD;Samlan,RobinA;Flint,PaulW
研究设计和设置高场电压（HV）和低场电压（LV）结合极性变化，以确定在5种不同条件下的转染：1）无EP（EP[-]），2）HV+LV（HL），3）HV+LV后HV+ LV，极性无变化（HLHL单向），4）HV+LV，然后是HV+LV，极性相反（HLHL双向），5）HV+LV，然后是相反极性的LV结果HLL双向序列表达效果最好，个体间差异小，表达周期长。除了重复的高电压序列，EP参数不太可能诱导细胞损伤或inflammation.ConclusionHLL双向电穿孔基因传递产生高的转染率与有限的tissue traum. Significance双向EP提供了一个安全和高效的方法治疗基因传递到骨骼肌。© 2006美国耳鼻咽喉头颈外科基金会学会。All rights reserved.
ObjectiveThe impact of polarity change on the efficiency of in vivo electroporative (EP) gene transfection was assessed in rat laryngeal muscle.Study Design and SettingHigh (HV) and low field voltage (LV) were combined with polarity changes to determine transfection in 5 different conditions: 1) without EP (EP[-]), 2) HV+LV (HL), 3) HV+LV followed by HV+LV with no change in polarity (HLHL unidirectional), 4) HV+LV followed by HV+LV with opposite polarity (HLHL bidirectional), 5) HV+LV followed by LV with opposite polarity (HLL bidirectional).ResultsHLL bidirectional sequence showed the best result with less interindividual variability and extended expression period. With the exception of repeated high voltage sequences, EP parameters were not likely to induce cell injury or inflammation.ConclusionHLL bidirectional electroporative gene delivery produces high transfection rates with limited tissue trauma.SignificanceBidirectional EP provides a safe and highly efficient method for therapeutic gene delivery into skeletal muscle. © 2006 American Academy of Otolaryngology-Head and Neck Surgery Foundation. All rights reserved.