The stabilization of electrospun chitosan nanofibers by reversible acylation
The stabilization of electrospun chitosan nanofibers by reversible acylation
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DOI：
10.1007/s10570-014-0306-3
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发表时间：
2014-06
期刊：
影响因子：
5.7
通讯作者：
Chaoxi Wu;H. Su;Shunqing Tang;J. Bumgardner
中科院分区：
文献类型：
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作者：
Chaoxi Wu;H. Su;Shunqing Tang;J. Bumgardner
静电纺丝制备的壳聚糖纳米纤维受到酸纺丝溶液中的酸性阴离子的污染，导致纳米纤维在水溶液中的不稳定性，而传统的纤维处理方法也会导致纳米结构的恶化。在这里，我们展示了一种新颖的方法来去除酸性阴离子，同时充分保存纳米纤维结构。纺丝后的纳米纤维首先被可逆酰化保护（稳定）。其次，通过水解去除污染物；最后，酰化发生逆转。化学分析表明，酸阴离子被去除，酰基被接枝和去除。形态学分析表明，可逆酰化纤维的直径<150 nm，在水溶液中浸泡后仍保持纳米纤维结构。膜也与培养的骨细胞相容。制备的纯壳聚糖纳米纤维在水溶液中具有良好的稳定性，在生物医学领域具有广阔的应用前景。
Chitosan nanofibers fabricated by electrospinning are contaminated by acidic anions from the acid spinning solution, leading to instability of the nanofibers in aqueous solutions, and the traditional fiber treatment method will also lead to the deterioration of the nanostructure. Here we demonstrate a novel approach to removing the acidic anions with full preservation of the nanofibrous structure. The as-spun nanofibers are first protected (stabilized) by reversible acylation. Second, contaminants are then eliminated by hydrolysis; finally, acylation is reversed. Chemical analysis showed the removal of the acidic anions and the graft and removal of acyl groups. Morphological analysis showed that the reversibly acylated fibers had diameters <150 nm and nanofiber structure was maintained after immersion in aqueous solution. The membranes also were compatible with bone cells in culture. The resultant pure chitosan nanofibers show excellent stability in aqueous solution and exhibit broad potential in biomedical applications.