Sequential Washing with Electrolyzed Alkaline and Acidic Water Effectively Removes Pathogens from Metal Surfaces.
Sequential Washing with Electrolyzed Alkaline and Acidic Water Effectively Removes Pathogens from Metal Surfaces.
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DOI：
10.1371/journal.pone.0156058
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Nishida N
中科院分区：
文献类型：
--
作者：
Nakano Y;Akamatsu N;Mori T;Sano K;Satoh K;Nagayasu T;Miyoshi Y;Sugio T;Sakai H;Sakae E;Ichimiya K;Hamada M;Nakayama T;Fujita Y;Yanagihara K;Nishida N
从再处理手术器械中去除病原微生物对于预防医源性感染至关重要。一些细菌可以在医疗设备上形成持久的生物膜。朊病毒污染的非一次性设备也对手术患者构成严重风险。对来自内窥镜和其他仪器（例如高分辨率相机）的朊病毒的有效消毒仍然存在问题，因为这些仪器不耐受侵蚀性化学或热处理。在此，我们开发了一种新的洗涤系统，该系统使用电解产生的碱性和酸性水。据报道，含有HCl和HOCl作为活性物质的电解酸性水是一种有效的消毒剂。将0.15%NaCl溶液电解并立即用于用碱性水（pH 11.9）超声处理洗涤生物污染的不锈钢模型系统，然后用酸性水（pH 2.7）不超声处理洗涤。两种细菌物种（金黄色葡萄球菌和铜绿假单胞菌）和真菌（白色念珠菌）被有效地去除或灭活的清洗过程。此外，该过程有效地从不锈钢表面去除或灭活朊病毒。该清洗系统将潜在地用于神经内窥镜等临床设备的消毒，因为电解水对患者和设备都是温和的，并且是环境友好的。
Removal of pathogenic organisms from reprocessed surgical instruments is essential to prevent iatrogenic infections. Some bacteria can make persistent biofilms on medical devices. Contamination of non-disposable equipment with prions also represents a serious risk to surgical patients. Efficient disinfection of prions from endoscopes and other instruments such as high-resolution cameras remains problematic because these instruments do not tolerate aggressive chemical or heat treatments. Herein, we develop a new washing system that uses both the alkaline and acidic water produced by electrolysis. Electrolyzed acidic water, containing HCl and HOCl as active substances, has been reported to be an effective disinfectant. A 0.15% NaCl solution was electrolyzed and used immediately to wash bio-contaminated stainless steel model systems with alkaline water (pH 11.9) with sonication, and then with acidic water (pH 2.7) without sonication. Two bacterial species (Staphylococcus aureus and Pseudomonas aeruginosa) and a fungus (Candida albicans) were effectively removed or inactivated by the washing process. In addition, this process effectively removed or inactivated prions from the stainless steel surfaces. This washing system will be potentially useful for the disinfection of clinical devices such as neuroendoscopes because electrolyzed water is gentle to both patients and equipment and is environmentally sound.