Survivin-targeted nanoparticles for pancreatic tumor imaging in mouse model
Survivin-targeted nanoparticles for pancreatic tumor imaging in mouse model
复制标题
生存素靶向纳米颗粒用于小鼠模型胰腺肿瘤成像
DOI：
10.1016/j.nano.2016.02.008
复制
发表时间：
2016-08-01
影响因子：
5.4
通讯作者：
Li, Donghui
中科院分区：
文献类型：
--
作者：
Wang, Zhongqiu;Tong, Mingmin;Li, Donghui
We investigated the potential of targeting survivin, an inhibitor of apoptosis, in visualize pancreatic tumor in mouse model using targeted magnetic nanoparticles (MNPs) and magnetic resonance imaging (MRI). Chitosan-coated MNPS and survivin antisense oligonucleotide(ASON) were conjugated to give Sur-MNPs. Accumulations of targeted, non-targeted nanoparticles or nonsense oligonucleotide-MNPs (NSON-MNPs) in the liver, spleen, kidney and tumors were determined. Targeted nanoparticles were highly accumulated in BxPC-3 cells but not in non-cancer cells. In vivo MRI showed a significant T-2 signal reduction in tumors of mice injected with targeted nanoparticles but slight signal change in tumors of mice injected with non-targeted nanoparticles or NSON-MNPs. Prussian blue staining demonstrated highly accumulated Sur-MNPs in tumor mass compared with normal pancreatic, kidney and liver tissues. Our data show that the MNPs functionalized with ASON lead to the targeted localization in pancreatic tumors. Survivin targeted nanoparticles could be used for detection of pancreatic tumors. (C) 2016 Elsevier Inc. All rights reserved.
