Detection in bovine adrenal cortex of a lipoidal substance that yields pregnenolone upon treatment with alkali.
Detection in bovine adrenal cortex of a lipoidal substance that yields pregnenolone upon treatment with alkali.
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检测牛肾上腺皮质中的类脂物质，经碱处理后可产生孕烯醇酮。
DOI：
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发表时间：
1977
影响因子：
11.1
通讯作者：
S. Lieberman
中科院分区：
文献类型：
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作者：
R. Hochberg;L. Bandy;L. Ponticorvo;S. Lieberman
牛肾上腺皮质组织含有孕烯醇酮（3 - β -羟基-孕-5-烯-20-酮）的脂质衍生物，其中的游离类固醇可通过碱处理释放出来。这种物质存在的证据来自于对组织的非极性萃取物的检查，其中孕烯醇酮及其硫酸盐已被色谱法除去。用碱处理非极性馏分，然后穷举色谱分析，检测孕烯醇酮。通过气相色谱/质谱法和双同位素方法鉴定了类固醇。定量分析表明，三种形式的孕烯醇酮在牛肾上腺皮质组织中以以下量（杯/公斤）存在：脂质衍生物，290；游离类固醇，435分；硫酸根，65。由于孕烯醇酮唯一已知的代谢功能是作为类固醇激素的前体，这些发现对类固醇激素的生物化学具有深远的意义。
Bovine adrenal cortical tissue contains a lipoidal derivative of pregnenolone (3beta-hydroxy-pregn-5-en-20-one) from which the free steroid can be liberated by treatment with alkali. Evidence for the presence of such an entity comes from examination of a nonpolar extract of tissue from which pregnenolone and its sulfate had been removed by chromatography. Treatment of the nonpolar fraction with alkali followed by exhaustive chromatographic analysis led to the detection of pregnenolone. The steroid was identified by both gas chromatography/mass spectrometry and double isotope procedures. Quantitative analysis indicated that the three forms of pregnenolone are present in bovine adrenal cortical tissue in the following amounts (mug/kg): lipoidal derivative, 290; free steroid, 435; and sulfate, 65. Because the only known metabolic function of pregnenolone is to serve as a precursor of the steroid hormones, these findings have far-reaching implications for steroid hormone biochemistry.