Sensitivity of different cell lines and of different phases in the cell cycle to hyperthermia.
Sensitivity of different cell lines and of different phases in the cell cycle to hyperthermia.
复制标题
不同细胞系和细胞周期不同阶段对热疗的敏感性。
作者：
B. Bhuyan;K. J. Day;C. E. Edgerton;O. Ogunbase
比较了不同细胞系（中国仓鼠卵巢、HeLa、L1210和P388）对43度的敏感性中国仓鼠卵巢和HeLa细胞对温度的敏感性远低于L1210或P388细胞。即使HeLa和L1210细胞在相同的培养基中生长，这种差异也持续存在，这表明这是细胞系之间的固有差异。在所有的细胞系中，7天的L1210腹水，通过转移在小鼠中维持，是最敏感的。与4天的L1210腹水或培养中的细胞相比，这些细胞的更大的敏感性可以通过它们的生长阶段的差异来解释。与指数生长的4天腹水或培养中的细胞相比，7天腹水细胞将处于静止生长。测定了中国仓鼠卵巢细胞在细胞周期不同阶段的温度敏感性。中、晚期S期细胞比有丝分裂期和早期S期细胞敏感，G1和G2期细胞敏感性最低。在部分同步化培养中，随着S期细胞比例的增加，L1210细胞的热敏感性增加。
The sensitivity of different cell lines (Chinese hamster ovary, HeLa, L1210, and P388) to 43 degrees was compared Chinese hamster ovary and HeLa cells were much less temperature sensitive than were L1210 or P388 cells. This difference persisted even when HeLa and L1210 cells were grown in the same medium and suggested that this was an inherent difference between the cell lines. Of all the cell lines, 7-day L1210 ascites, maintained by transfer in mice, were the most sensitive. The greater sensitivity of these cells, as compared to 4-day L1210 ascites or cells in culture, may be explained by the difference in their growth stages. The 7-day ascites cells would be in stationary growth as compared to the exponentially growing 4-day ascites or the cells in culture. The temperature sensitivity of Chinese hamster ovary cells in different parts of the cell cycle was determined. Mid and late-S-phase cells were more sensitive than cells in mitosis or early S; G1 and G2 cells were the least sensitive. In a partially synchronized culture, the heat sensitivity of L1210 cells increased with increased percentage of cells in S phase.