A unique virulence factor for proliferation and dwarfism in plants identified from a phytopathogenic bacterium
A unique virulence factor for proliferation and dwarfism in plants identified from a phytopathogenic bacterium
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DOI：
10.1073/pnas.0813038106
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发表时间：
2009-04-14
影响因子：
11.1
通讯作者：
Namba, Shigetou
中科院分区：
文献类型：
--
作者：
Hoshi, Ayaka;Oshima, Kenro;Namba, Shigetou
农业科学中最重要的主题之一是确定与植物病害有关的毒力因子。在这里，我们表明，单一的毒力因子，天谷-苏诱导物(Tengu)，诱导丛枝病和矮化，是植物病原菌-植原体的一种小分泌蛋白。当tengu在烟草植株中表达时，这些植物表现出丛枝病和矮化症状，这是植原体感染的典型症状。转Tengu基因的拟南芥品系表现出类似的症状，证实了Tengu的表达对植物的影响。虽然植原体的定位仅限于韧皮部，但免疫组织化学分析发现顶芽中存在Tengu蛋白，表明Tengu是从韧皮部运输到其他细胞的。基因芯片分析表明，与转GUS基因的对照植株相比，Tengu转基因植株中生长素反应基因的表达显著下调。这些结果表明，天谷抑制生长素相关的途径，从而影响植物的发育。
One of the most important themes in agricultural science is the identification of virulence factors involved in plant disease. Here, we show that a single virulence factor, tengu-su inducer (TENGU), induces witches' broom and dwarfism and is a small secreted protein of the plant-pathogenic bacterium, phytoplasma. When tengu was expressed in Nicotiana benthamiana plants, these plants showed symptoms of witches' broom and dwarfism, which are typical of phytoplasma infection. Transgenic Arabidopsis thaliana lines expressing tengu exhibited similar symptoms, confirming the effects of tengu expression on plants. Although the localization of phytoplasma was restricted to the phloem, TENGU protein was detected in apical buds by immunohistochemical analysis, suggesting that TENGU was transported from the phloem to other cells. Microarray analyses showed that auxin-responsive genes were significantly down-regulated in the tengu-transgenic plants compared with GUS-transgenic control plants. These results suggest that TENGU inhibits auxin-related pathways, thereby affecting plant development.