An oncolytic vaccinia virus expressing the human sodium iodine symporter prolongs survival and facilitates SPECT/CT imaging in an orthotopic model of malignant pleural mesothelioma.
An oncolytic vaccinia virus expressing the human sodium iodine symporter prolongs survival and facilitates SPECT/CT imaging in an orthotopic model of malignant pleural mesothelioma.
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DOI：
10.1016/j.surg.2013.06.004
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发表时间：
2013-09
期刊：
影响因子：
3.8
通讯作者：
Fong, Yuman
中科院分区：
文献类型：
--
作者：
Belin, Laurence J.;Ady, Justin W.;Lewis, Christina;Marano, Drew;Gholami, Sepideh;Mojica, Kelly;Eveno, Clarisse;Longo, Valerie;Zanzonico, Pat B.;Chen, Nanhai G.;Szalay, Aladar A.;Fong, Yuman
这项原创性工作的目的是研究表达人钠碘转运蛋白（hNIS）的溶瘤牛痘病毒对恶性胸膜间皮瘤（MPM）的病毒治疗和有效治疗提供真实的时间监测的能力。在体外测定GLV-1h 153对所有组织学亚型的间皮瘤细胞系的增殖和细胞毒作用。通过标准病毒空斑试验检查病毒复制。在无胸腺裸鼠中产生原位MPM异种移植物，并用胸膜内GLV-1h 153处理，并评估对肿瘤负荷和存活的影响。原位肿瘤在131 I给药后也在SPECT/CT上成像。GLV-1h 153以时间和浓度依赖性方式感染并杀死所有细胞系。病毒复制证明在4天内滴度增加超过2.5 log。原位MPM异种移植物的胸膜内治疗导致治疗后一周肿瘤负荷的显著降低和存活率的改善。原位异种移植物的感染是治疗性的，并且通过感染组织中hNIS的表达通过131 I-SPECT/CT促进监测。我们的研究结果表明，GLV-1h 153是一个有前途的治疗剂MPM，值得进一步研究。
The purpose of this original work is to examine the ability of an oncolytic vaccinia virus expressing the human sodium iodine transporter (hNIS) to provide real time monitoring of viral therapy and effective treatment of malignant pleural mesothelioma (MPM). Infectivity and cytotoxic effect of GLV-1h153 on mesothelioma cell lines of all histologic subtypes was assayed in vitro. Viral replication was examined by standard viral plaque assay. Orthotopic MPM xenografts were generated in athymic nude mice and treated with intrapleural GLV-1h153 and assessed for effect on tumor burden and survival. Orthotopic tumors were also imaged on SPECT/CT after 131I administration. GLV-1h153 infected and killed all cell lines in a time and concentration dependent manner. Viral replication demonstrated over a 2.5 log increase in titer over 4 days. Intrapleural treatment of orthotopic MPM xenografts resulted in a significant reduction in tumor burden one week after treatment and an improvement in survival. Infection of orthotopic xenografts was both therapeutic and facilitated monitoring by 131I-SPECT/CT via expression of hNIS in infected tissue. Our results suggest GLV-1h153 is a promising therapeutic agent for MPM and warrants further investigation.
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影响因子：
11.2
作者：
Zhang, Qian;Yu, Yong A.;Szalay, Aladar A.
通讯作者：
Szalay, Aladar A.
影响因子：
5.7
作者：
Silberhumer GR;Brader P;Wong J;Serganova IS;Gönen M;Gonzalez SJ;Blasberg R;Zamarin D;Fong Y
通讯作者：
Fong Y
影响因子：
7.4
作者：
Haddad D;Chen NG;Zhang Q;Chen CH;Yu YA;Gonzalez L;Carpenter SG;Carson J;Au J;Mittra A;Gonen M;Zanzonico PB;Fong Y;Szalay AA
通讯作者：
Szalay AA
影响因子：
3.5
作者：
Adusumilli, Prasad S.;Stiles, Brendon M.;Fong, Yuman
通讯作者：
Fong, Yuman
影响因子：
4.2
作者：
Kelly, Kaitlyn J.;Woo, Yanghee;Fong, Yuman
通讯作者：
Fong, Yuman