Nano-TiO2 enhanced bioaccumulation and developmental neurotoxicity of bisphenol a in zebrafish larvae
Nano-TiO2 enhanced bioaccumulation and developmental neurotoxicity of bisphenol a in zebrafish larvae
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纳米TiO2增强双酚A在斑马鱼幼虫中的生物蓄积和发育神经毒性
DOI：
10.1016/j.envres.2020.109682
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发表时间：
2020-08-01
影响因子：
8.3
通讯作者：
Zhou, Bingsheng
中科院分区：
文献类型：
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作者：
Fu, Juanjuan;Guo, Yongyong;Zhou, Bingsheng
纳米二氧化钛（n-TiO 2）可提高水相中共存有机毒物的生物利用度和毒性。父母共同暴露于n-TiO 2和双酚A（BPA）可能会产生发育神经毒性在未暴露的斑马鱼后代。然而，关于在早期发育阶段共同暴露后对仔鱼的发育神经毒性，仍有待探讨。在本研究中，受精的斑马鱼卵暴露于TiO 2纳米颗粒（100 μ g/L），BPA（1，4和20 μ g/L）或其二元混合物，直到6天后受精（dpf）。各处理组幼虫的孵化率、畸形率、存活率和体重均无明显变化。然而，n-TiO 2显着增加BPA的身体负担，在4和20 μ g/L的共同暴露组，抑制神经发育标记基因（微管蛋白，mbp和syn 2a）的表达以及运动行为。目前的研究结果表明，n-TiO 2通过促进BPA的生物累积和生物利用度，增强了共暴露斑马鱼幼体的发育神经毒性和失活运动，这表明在早期发育阶段共暴露后与亲代共暴露具有相似的发育神经毒性毒性风险。
The titanium dioxide nanoparticles (n-TiO2) could enhance the bioavailability and toxicity of the coexisted organic toxicants in aquatic phase. Parental co-exposure to n-TiO2 and bisphenol A (BPA) could generate developmental neurotoxicity in unexposed zebrafish offspring. However, it remains unexplored regarding the developmental neurotoxicity in larvae fish after co-exposure during the early developmental stage. In present study, fertilized zebrafish eggs were exposed to TiO2 nanoparticles (100 mu g/L), BPA (1, 4 and 20 mu g/L) or their binary mixtures until 6 days post fertilization (dpf). No significant change was observed in hatching, malformation, survival and weight of the larvae among all groups. However, n-TiO2 significantly increased the body burden of BPA in the 4 and 20 mu g/L co-exposure groups, depressed expression of neurodevelopment marker genes (al-tubulin, mbp and syn2a) as well as the locomotor behavior. The current results indicate that n-TiO2 could strengthen the developmental neurotoxicity and inactive locomotion in co-exposed zebrafish larvae by promoting the bioaccumulation and bioavailability of BPA, which highlighted the similar toxic risks of developmental neurotoxicity after co-exposure at early developmental stage to that of the parental co-exposure.