Phosphorylation-dependent kinase-substrate cross-linking.
Phosphorylation-dependent kinase-substrate cross-linking.
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DOI：
10.1002/anie.200905244
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发表时间：
2010-02-22
影响因子：
16.6
通讯作者：
Pflum, Mary Kay H.
中科院分区：
文献类型：
--
作者：
Suwal, Sujit;Pflum, Mary Kay H.
Kinases catalyze protein phosphorylation to regulate signal transduction in a cell. Thus, the identification of substrates of a particular kinase is a fundamental step toward understanding the signaling cascades in normal and disease states. With over 500 known kinases and a paucity of tools available, the identification of genuine kinase substrates has been difficult.[1–7] New approaches to detect substrates are needed to fully characterize cell-signaling networks.Adenosine 5′-triphosphate (ATP, 1; Scheme 1a) is the universal cosubstrate responsible for protein phosphorylation. During phosphorylation, the γ phosphate group of ATP is transferred to serine, threonine, and tyrosine residues on eukaryotic protein substrates. Recently, we demonstrated that cellular kinases promiscuously accept ATP analogues with γ-phosphate modifications. Specifically, we coupled ATP analogues modified at the γ-phosphate position with either a dansyl [8] or a biotin [9] group (see derivative 2in Scheme 1a) with cellular kinases to conveniently label peptides and proteins with the dansyl or biotin group for subsequent analysis by mass spectrometry (MS) or gel electrophoresis. Kinase-catalyzed phosphoprotein labeling is a useful enzymatic approach to attach a desired functional probe to a substrate protein.
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影响因子：
2.9
作者：
Xiong, Y;Bernardi, D;Radominska-Pandya, A
通讯作者：
Radominska-Pandya, A
影响因子：
15
作者：
Maly, DJ;Allen, JA;Shokat, KM
通讯作者：
Shokat, KM
DOI：
10.1073/pnas.0708966105
发表时间：
2008-02-05
影响因子：
11.1
作者：
Blethrow, Justin D.;Glavy, Joseph S.;Shokat, Kevan M.
通讯作者：
Shokat, Kevan M.
影响因子：
3.2
作者：
Green, Keith D.;Pflum, Mary Kay H.
通讯作者：
Pflum, Mary Kay H.
影响因子：
15
作者：
Statsuk, Alexander V.;Maly, Dustin J.;Shokat, Kevan M.
通讯作者：
Shokat, Kevan M.