Cytotoxicity and genotoxicity of pulp capping materials in two cell lines
Cytotoxicity and genotoxicity of pulp capping materials in two cell lines
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DOI：
10.1111/j.1365-2591.2008.01506.x
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发表时间：
2009-03-01
影响因子：
5
通讯作者：
Schmalz, G.
中科院分区：
文献类型：
--
作者：
Camargo, S. E. A.;Camargo, C. H. R.;Schmalz, G.
本研究的目的是评价蓖麻油豆水泥（COB）材料的细胞毒性和遗传毒性，并与常用的髓盖材料进行比较。在91.6 mm(2)样品表面mL（-1）的培养基中提取COB、氢氧化钙（Hydro C）和矿物三氧化骨料（白色和灰色MTA）标本。将转染的人髓细胞（tHPCs）暴露于提取物的稀释液中1小时，以H2DCF-DA为染料，用流式细胞术（FACS）检测活性氧（ROS）的生成。暴露24小时后，采用结晶紫法测定thpc的存活率。通过对V79细胞微核形成的遗传毒性，以及对材料提取物对正常细胞周期的改变进行FACS分析。在目前的实验条件下，仅用Hydro C提取物检测到明显的细胞毒作用。两种MTA制剂诱导细胞数量的显著减少。相反，COB提取物对细胞增殖有轻微的诱导作用。Hydro C提取物使ROS的产生增加了两倍，而其他被测材料则无效。检测的任何材料均未检测到微核数量的增加；在G1和G2中，Hydro C略微增加细胞数量。COB和两种MTA制剂对细胞存活或ROS产生没有负面影响，因此可以进一步考虑用于髓盖研究。
The aim of this study was to evaluate the cytotoxicity and genotoxicity of the new castor oil bean cement (COB) material in comparison to commonly used pulp capping materials.Specimens of COB, calcium hydroxide (Hydro C), and mineral trioxide aggregate (white and gray MTA) were extracted in culture medium (91.6 mm(2) sample surface mL(-1)). Transfected human pulp cells (tHPCs) were exposed to dilutions of the extracts for 1 h, and the generation of reactive oxygen species (ROS) was determined by flow cytometry (FACS) using H2DCF-DA as a dye. Survival of tHPCs was measured photometrically using a crystal violet assay after a 24-h exposure period. Genotoxicity as indicated by the formation of micronuclei in V79 cells, and the modification of the normal cell cycle by extracts of the materials was analysed by FACS.Clear cytotoxic effects were detected only with extracts of Hydro C under the current experimental conditions. The two MTA preparations induced an insignificant reduction in the number of cells. In contrast, the extracts of COB slightly induced cell proliferation. Extracts of Hydro C caused a twofold increase in ROS production, whilst the other tested materials were ineffective. An increase in the number of micronuclei was not detected with any material tested; Hydro C slightly increased the number of cells in G1 and G2.The COB and the two MTA preparations did not negatively influence cell survival or ROS production and may thus be further considered for pulp capping studies.