An improved type of 'Productmeter', a differential gas-volumeter, and its application to measuring photosynthesis of seaweeds
An improved type of 'Productmeter', a differential gas-volumeter, and its application to measuring photosynthesis of seaweeds
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改进型“Productmeter”、差动气体体积计及其在测量海藻光合作用中的应用
DOI：
10.1002/mrd.20250
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发表时间：
1986
影响因子：
2.5
通讯作者：
Y. Yokohama
中科院分区：
文献类型：
--
作者：
Y. Yokohama
有许多关于使用RNA-DNA寡核苷酸（嵌合体）在体外和体内纠正点突变的报道，包括人载脂蛋白E基因（ApoE）。尽管没有针对靶向的选择，但已报道了高效转化。虽然主要用于逆转基因治疗应用中的有害突变，但这种方法的成功使用将有可能极大地促进小鼠和其他物种中确定突变的产生。我们试图通过将嵌合体显微注射到单细胞小鼠卵子的原核中，在小鼠ApoEgene中产生点突变。移植显微注射的卵子后，我们分析了139个E12.5胚胎，但没有获得成功转化的证据。摩尔Reprod. Dev. 71：140-144，2005.© 2005 Wiley利斯公司
There are numerous reports of the use of RNA–DNA oligonucleotides (chimeraplasts) to correct point mutations in vitro and in vivo, including the human apolipoprotein E gene (ApoE). Despite the absence of selection for targeting, high efficiency conversion has been reported. Although mainly used to revert deleterious mutations for gene therapy applications, successful use of this approach would have the potential to greatly facilitate the production of defined mutations in mice and other species. We have attempted to create a point mutation in the mouseApoEgene by microinjection of chimeraplast into the pronuclei of 1‐cell mouse eggs. Following transfer of microinjected eggs we analysed 139 E12.5 embryos, but obtained no evidence for successful conversion. Mol. Reprod. Dev. 71: 140–144, 2005. © 2005 Wiley‐Liss, Inc.