Influence of different types of contact hypersensitivity on imiquimod-induced psoriasis-like inflammation in mice
Influence of different types of contact hypersensitivity on imiquimod-induced psoriasis-like inflammation in mice
复制标题
不同类型接触超敏反应对咪喹莫特诱导的小鼠银屑病样炎症的影响
DOI：
10.3892/mmr.2016.5299
复制
发表时间：
2016-07-01
影响因子：
3.4
通讯作者：
Liu, Xiaoming
中科院分区：
文献类型：
--
作者：
Bai, Shuang;Zhang, Zhenying;Liu, Xiaoming
目前认为银屑病和过敏性接触性皮炎（ACD）是不同的疾病;然而，它们具有临床相似性。辅助性T细胞17（Th 17）参与这些疾病提供了一个新的机会来研究它们之间的关系。本研究的目的是确定是否相同或重叠的炎症途径参与这两种疾病，以及不同类型的ACD对银屑病的影响。建立了Th 1或Th 2型接触性超敏反应（CHS）联合咪喹莫特（IMQ）诱导的银屑病样炎症的复合小鼠模型，以模拟ACD和银屑病的特征。采用组织学、免疫组化及血清和组织中细胞因子检测等方法，比较单用IMQ或IMQ联合Th 1、Th 2型CHS治疗小鼠的差异。与经IMQ治疗的小鼠或经IMQ治疗的Th 1型CHS小鼠相比，经IMQ治疗的Th 2型CHS小鼠表现出更严重的银屑病样炎症，表皮厚度增加，真皮中的浸润细胞增加。在背部皮损中检测到高水平的白细胞介素（IL）-17、IL-22、IL-23、TNF-α和ROR γ t的mRNA表达。另外，在IMQ处理的小鼠中检测到高水平的IL-17和IL-22，其与Th 2型CHS组合。单独用IMQ治疗的小鼠，以及IMQ治疗与Th 1型CHS联合治疗的小鼠在背部皮肤中具有相当的银屑病样炎症反应。总之，这些数据表明，Th 2型CHS通过IL-23/IL-17轴加重了经MQ治疗的小鼠银屑病炎症。Th 17细胞及其相关通路可能与ACD和银屑病相关。因此，银屑病患者应避免接触可能诱发Th 2极化的特定致敏物，如香水和橡胶制品。
It is currently believed that psoriasis and allergic contact dermatitis ( ACD) are different diseases; however, they share clinical similarities. The involvement of T helper 17 ( Th17) cells in these disorders provides a novel opportunity to investigate the relationship between them. The present study aimed to determine whether the same or overlapping inflammatory pathways are involved in the two diseases, and the influence of different types of ACD on psoriasis. Compound mouse models of Th1 or Th2-type contact hypersensitivity ( CHS) combined with imiquimod ( IMQ)-induced psoriasis-like inflammation were established, in order to mimic the characteristics of ACD and psoriasis. Histopathology, immunohistochemistry and cytokine detection in blood serum and tissues were used to compare the differences between the mice treated with IMQ alone or IMQ combined with Th1 and Th2-type CHS. As compared with the IMQ-treated mice or IMQ-treated Th1-type CHS mice, the mice with Th2-type CHS treated with IMQ exhibited more serious psoriasis-like inflammation with increased epidermal thickness and infiltrating cells in the derma. High mRNA expression levels of interleukin ( IL)-17, IL-22, IL-23, TNF-alpha and ROR gamma t were detected in back skin lesions. Additionally, high levels of IL-17 and IL-22 in blood serum were detected in IMQ-treated mice combined with Th2-type CHS. The mice treated with IMQ alone, and IMQ treatment combined with Th1-type CHS had a comparable psoriasis-like inflammatory response in the back skin. In conclusion, these data demonstrate that Th2-type CHS exacerbated the IMQ-treated psoriatic inflammation of mice via the IL-23/IL-17 axis. Th17 cells and associated pathways may link ACD and psoriasis. Therefore, patients with psoriasis should avoid contact with specific sensitizers, such as fragrance and rubber products, which may induce Th2 polarization.