Telomerase reverse transcriptase mRNA expression and telomerase activity in hepatocellular carcinoma
Telomerase reverse transcriptase mRNA expression and telomerase activity in hepatocellular carcinoma
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DOI：
10.1007/s005350050220
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发表时间：
1999-02
影响因子：
6.3
通讯作者：
K. Nagao;M. Tomimatsu;H. Endo;H. Hisatomi;K. Hikiji
中科院分区：
文献类型：
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作者：
K. Nagao;M. Tomimatsu;H. Endo;H. Hisatomi;K. Hikiji
人端粒酶逆转录酶（Human telomerase reverse transcriptase，hTERT）是人端粒酶的催化亚基。为探讨hTERTmRNA在肝细胞癌（HCC）中的临床意义，我们研究了人HCC和非HCC组织中端粒酶活性与hTERTmRNA的关系。hTERTmRNA在19例肝癌组织中的阳性率为89.47%（17/19），在19例癌旁肝组织中的阳性率为21.05%（4/19）。19例肿瘤组织中17例（89.47%）端粒酶活性阳性，19例非肿瘤组织中4例（21.05%）端粒酶活性阳性。端粒酶阳性的组织中检测到hTERTmRNA，端粒酶阴性的组织中未检测到hTERTmRNA。本研究中hTERTmRNA表达与人端粒酶活性之间的相关性表明hTERTmRNA可用于诊断癌症。另外，端粒酶的产生可能受hTERTmRNA的调控，当hTERTmRNA阳性时，慢性肝病的非癌肝组织获得HCC的可能性很大。
Human telomerase reverse transcriptase (hTERT) has been identified as the catalytic subunit of human telomerase. To clarify the clinical significance of hTERT mRNA in hepatocellular carcinoma (HCC), we investigated the relationship between telomerase activity and hTERT mRNA in human HCC and non-HCC tissues. The hTERT mRNA was detected in 17 (89.47%) of 19 livers with HCC and in 4 (21.05%) of 19 noncancerous tissues from these livers. Telomerase activity was detected in 17 of the 19 tumor tissues (89.47%) and in 4 of the 19 nontumor tissues (21.05%). The hTERT mRNA was detected in all tissues that were telomerase-positive and it was undetected in all tissues that were telomerase-negative. The correlation between the expression of hTERT mRNA and human telomerase activity in this study indicates that hTERT mRNA could be useful to diagnose cancer. Also, as telomerase production may be under the control of hTERT mRNA, the possibility is great that noncancerous liver tissue with chronic liver diseases acquires HCC when the hTERT mRNA is positive.