EFFICIENT GENERATION OF ANTIBODIES TO ONCOPROTEINS BY USING SYNTHETIC PEPTIDE ANTIGENS
EFFICIENT GENERATION OF ANTIBODIES TO ONCOPROTEINS BY USING SYNTHETIC PEPTIDE ANTIGENS
复制标题
DOI：
10.1073/pnas.82.10.3400
复制
发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
CLINE, MJ
中科院分区：
文献类型：
--
作者：
TANAKA, T;SLAMON, DJ;CLINE, MJ
为了检测产生蛋白反应性抗肽抗体的效率，使用由逆转录病毒或细胞癌基因编码的35种肽来免疫兔子。肽（32）引发抗肽抗体，其中56%与其各自的癌蛋白反应。免疫肽的长度是产生与天然蛋白质反应的抗体的重要因素。在单个或几个氨基酸上不同的类似肽可以引起反应性明显不同的抗血清。
To examine the efficiency of generating protein-reactive antipeptide antibodies, 35 peptides encoded by retroviral or cellular oncogenes were used to immunize rabbits. Peptides (32) elicited antipeptide antibodies, of which 56% reacted with their respective oncoproteins. The length of the immunizing peptide was an important factor in generating antibodies reactive with native protein. Similar peptides differing in a single or a few amino acids could elicit antisera of markedly different reactivities.