Acute spinal cord injury could cause activation of autophagy in dorsal root ganglia
Acute spinal cord injury could cause activation of autophagy in dorsal root ganglia
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DOI：
10.1038/sc.2013.52
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发表时间：
2013-09-01
期刊：
影响因子：
2.2
通讯作者：
Tang, P.
中科院分区：
文献类型：
--
作者：
Hou, H.;Zhang, L.;Tang, P.
目的：背根神经节（Dorsal root ganglia, DRGs）在初级传入神经元的外周感觉机制中具有重要作用，被广泛用于研究细胞死亡、轴突再生、生长因子信号传递和疼痛机制等过程。方法：研究急性脊髓损伤大鼠模型不同时间点DRGs自噬的激活情况。结果：DRGs自噬标志物微管相关蛋白轻链3的表达在8 h后升高，3 d后达到峰值，7 d后逐渐降低。甲苯胺蓝染色证实急性脊髓损伤后，DRGs髓鞘随着时间的推移发生组织病理学改变，轴突肿胀，排列混乱，分布不均匀。结论：旨在恢复行为运动和感觉知觉的潜在治疗应针对DRGs的自噬过程。
Objectives: Dorsal root ganglia (DRGs) have an important role in the peripheral mechanism of sensation by primary afferent neurons, which are widely used to research the processes of cell death, axonal regeneration, signal transmission of growth factors and the mechanism of pain.Methods: In the present study, we investigated the activation of autophagy in DRGs in a rat model of acute spinal cord injury at different time points.Results: Expression of microtubule-associated protein light chain 3, a marker of autophagy was increased after 8 h in DRGs, peaked after 3 days, and then gradually decreased after 7 days. Furthermore, the toluidine blue staining has proven that after acute spinal cord injury, the myelin sheathes of DRGs undergo histopathological changes over time, with axonal swellings, disorderly arrangement and uneven distribution.Conclusion: Potential treatment aimed at recovery of behavioral locomotor and sensory perception should target the process of autophagy in DRGs.