Cell cycling and cell enlargement in developing leaves of Arabidopsis
Cell cycling and cell enlargement in developing leaves of Arabidopsis
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DOI：
10.1006/dbio.1999.9443
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发表时间：
1999-11-15
影响因子：
2.7
通讯作者：
Dengler, NG
中科院分区：
文献类型：
--
作者：
Donnelly, PM;Bonetta, D;Dengler, NG
细胞周期在植物发育中起着重要作用，包括：（1）器官形态建成，（2）组织内细胞增殖，（3）细胞分化。在这项研究中，我们使用一个细胞周期蛋白：：β-葡萄糖醛酸酶报告构建的特点，在野生型发育过程中的模式遗传生物拟南芥（哥伦比亚）在这些水平的细胞周期的空间和时间模式。我们发现，在叶发育，叶片形成，一个关键的形态发生事件，是高度相关的本地化的细胞周期在原基边缘。然而，组织层是由不直接涉及边缘区的循环细胞的更扩散分布建立的。在叶片展开过程中，组织增殖表现出强烈的纵向梯度，具有基质体极性。组织层的增殖细胞分裂的模式不同：细胞周期的栅栏叶肉前体相比，延长相邻的表皮层的铺面细胞，细胞退出周期在不同的特征尺寸。细胞分裂直接相关的气孔和维管组织的形成，从他们各自的前体发生在整个叶伸展期间，因此，不同的组织模式反映了叠加的周期相关的细胞分化更一般的组织增殖。我们的研究结果表明，与叶形态发生，组织特异性的细胞增殖模式，细胞分化的细胞周期同时发生在叶发育过程中，并建议独特的调节途径可能在每个级别上运行。(C)北京：科学出版社.
Cell cycling plays an important role in plant development, including: (1) organ morphogenesis, (2) cell proliferation within tissues, and (3) cell differentiation. In this study we use a cyclin::beta-glucuronidase reporter construct to characterize spatial and temporal patterns of cell cycling at each of these levels during wild-type development in the model genetic organism Arabidopsis thaliana (Columbia). We show that a key morphogenetic event in leaf development, blade formation, is highly correlated with localized cell cycling at the primordium margin. However, tissue layers are established by a more diffuse distribution of cycling cells that does not directly involve the marginal zone. During leaf expansion, tissue proliferation shows a strong longitudinal gradient, with basiplastic polarity. Tissue layers differ in pattern of proliferative cell divisions: cell cycling of palisade mesophyll precursors is prolonged in comparison to that of pavement cells of the adjacent epidermal layers, and cells exit the cycle at different characteristic sizes. Cell divisions directly related to formation of stomates and of vascular tissue from their respective precursors occur throughout the period of leaf extension, so that differing tissue patterns reflect superposition of cycling related to cell differentiation on more general tissue proliferation. Our results indicate that cell cycling related to leaf morphogenesis, tissue-specific patterns of cell proliferation, and cell differentiation occurs concurrently during leaf development and suggest that unique regulatory pathways may operate at each level. (C) 1999 Academic Press.