DNA Aptamers that Bind to PQQGDH as an Electrochemical Labeling Tool
DNA Aptamers that Bind to PQQGDH as an Electrochemical Labeling Tool
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DOI：
10.1002/elan.200804559
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发表时间：
2009-06-01
期刊：
影响因子：
3
通讯作者：
Ikebukuro, Kazunori
中科院分区：
文献类型：
--
作者：
Osawa, Yuko;Takase, Madoka;Ikebukuro, Kazunori
我们筛选的DNA适体结合吡咯喹啉醌葡萄糖脱氢酶（PQOGDH）的电化学标记工具的发展。PQQGDH是一种优良的生物传感器信号放大酶。本研究以DNA适体为标记物，试图筛选出能与PQQGDH结合而不影响PQQGDH活性的DNA适体。在7轮筛选后，获得一种适体：“PGa4”。它与PQQGDH特异性结合，对葡萄糖脱氢酶（GDH）活性无影响。此外，通过PGa 4用PQQGDH标记的珠在加入葡萄糖时产生电流。因此，我们认为，PGa 4针对PQQGDH的适配子可能成为其强有力的标记工具，用于电化学生物传感器。
We screened DNA aptamers that bind to pyrroquinoline quinone glucose dehydrogenase (PQOGDH) for the development of an electrochemical labeling tool. PQQGDH is an excellent enzyme for the signal amplification of biosensors. We focused on DNA aptamers as labeling, agents and tried to select those DNA aptamers that bind to PQQGDH without affecting its crizvniatic activity. After 7 rounds of screening, one aptamer was obtained: 'PGa4'. It bound to PQQGDH with specificity and showed no effect on the glucose dehydrogenase (GDH) activity. Moreover, beads labeled with PQQGDH via PGa4 generated an electrical current upon glucose addition. Therefore, we believe that the PGa4 aptamer against PQQGDH may become it powerful labeling tool for electrochemical biosensors.