Effects of Curcumin on Lung Histopathology and Fungal Burden in a Mouse Model of Chronic Asthma and Oropharyngeal Candidiasis
Effects of Curcumin on Lung Histopathology and Fungal Burden in a Mouse Model of Chronic Asthma and Oropharyngeal Candidiasis
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DOI：
10.1016/j.arcmed.2011.01.011
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发表时间：
2011-02-01
影响因子：
7.7
通讯作者：
Karaman, Ozkan
中科院分区：
文献类型：
--
作者：
Karaman, Meral;Ayyildiz, Zeynep Arikan;Karaman, Ozkan
背景和目标。口咽念珠菌病（OPC）是目前治疗慢性哮喘最常见的局部副作用之一。需要具有较少副作用和逆转慢性变化的新治疗选择。姜黄素作为一种很有前途的抗真菌药物，有望成为治疗哮喘的替代药物。本研究旨在确定口服姜黄素对哮喘和OPC小鼠模型肺组织病理学、血清一氧化氮水平和真菌负荷的疗效。将35只BALB/c小鼠分为5组：I、II、III、IV（安慰剂）和V（对照）。除对照组外，所有组均用卵白蛋白致敏和激发。在建立慢性哮喘模型的基础上建立OPC模型。在用姜黄素、地塞米松、姜黄素-地塞米松组合和安慰剂治疗5天后评价肺组织学、血清一氧化氮水平和真菌负荷。光镜下观察肺组织学改变，包括上皮下平滑肌和上皮厚度、杯状细胞和肥大细胞数量。姜黄素组的所有组织学参数改善与地塞米松组相似。姜黄素和地塞米松-姜黄素组合在降低一氧化氮水平方面也与地塞米松一样有效。姜黄素治疗组的口腔真菌负荷显著低于地塞米松治疗组。在我们的研究中，我们证明了姜黄素给药可以减轻哮喘的病理变化，减少真菌负担。姜黄素可能具有治疗慢性哮喘和降低OPC频率的潜在作用。(C)2011年IMSS。爱思唯尔公司出版
Background and Aims. Oropharyngeal candidiasis (OPC) is one of the most common local side effects of current therapy in chronic asthma. New therapeutic options with fewer side effects and reverse chronic changes are needed. Curcumin, as a promising antiinflammatory and antifungal agent, could be a candidate of alternative therapy in asthma. This study aimed to determine the efficacy of orally administrated curcumin on lung histopathology, serum nitric oxide levels and fungal burden in a murine model of asthma and OPC.Methods. Thirty five BALB/c mice were divided into five groups: I, II, III, IV (placebo) and V (control). All groups except the control were sensitized and challenged with ovalbumin. OPC model was established after the model of chronic asthma. Lung histology, serum nitric oxide levels and fungal burden were evaluated after 5 days of treatment with curcumin, dexamethasone, curcumin-dexamethasone combination and placebo. Evaluation of lung histology included subepithelial smooth muscle and epithelial thickness and number of goblet and mast cells by using light microscopy.Results. All histological parameters improved in curcumin group similar to dexamethasone group. Curcumin and dexamethasone-curcumin combination were also as effective as dexamethasone on decreasing nitric oxide levels. Oral fungal burden was significantly lower in curcumin-treated group than dexamethasone.Conclusions. In our study we demonstrated that curcumin administration alleviates the pathological changes in asthma and decreases the fungal burden. Curcumin may have a potential effect on treating chronic asthma and decreasing the frequency of the OPC. (C) 2011 IMSS. Published by Elsevier Inc.