Effect of Hydroxycinnamic Acid Amides, Coumaroyl Tyramine and Coumaroyl Tryptamine on Biotic Stress Response in Arabidopsis
Effect of Hydroxycinnamic Acid Amides, Coumaroyl Tyramine and Coumaroyl Tryptamine on Biotic Stress Response in Arabidopsis
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DOI：
10.1007/s12374-021-09341-2
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发表时间：
2022-01-06
影响因子：
2.9
通讯作者：
Kim, Min Gab
中科院分区：
文献类型：
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作者：
Macoy, Donah Mary J.;Uddin, Shahab;Kim, Min Gab
香豆酰酪胺（CT）和香豆酰色胺（CTr）是中性羟基肉桂酸酰胺（HCAAs），在植物中积累响应病原体感染。在这项研究中，我们表明，接种假单胞菌pv。番茄DC 3000（Pto）和胡萝卜软腐欧文氏菌（Erwiniacarotovora carotovora，ECC）在接种后24 h增加了拟南芥叶片中CT的积累。CT和CTr均增加了病理相关1（PR 1）蛋白的积累。然而，CT和CTr没有显着的影响，在兼容和不兼容的植物-病原菌相互作用的抗活体营养型病原菌。尽管如此，我们的研究结果表明，CTr在增加植物对坏死营养型病原体ECC的敏感性方面起着关键作用。外源性应用CT和CTr增加胼胝质沉积的诱导在ECC的存在和不存在。在水杨酸诱导缺陷的拟南芥突变体Ein 2中检测到胼胝质沉积增加，但在乙烯信号转导突变体ein 2 -1和etr 1中检测到的程度较小。总体而言，我们的研究结果表明，乙烯信号与CT和CTR诱导的胼胝质沉积，并有助于植物防御病原体，而水杨酸是不需要这种反应。
Coumaroyl tyramine (CT) and coumaroyl tryptamine (CTr) are neutral hydroxycinnamic acid amides (HCAAs) that accumulate in plants in response to pathogen infection. In this study, we showed that inoculation of Pseudomonas syringae pv. tomato DC3000 (Pto) and Erwinia carotovora carotovora (ECC) increased the accumulation of CT in Arabidopsis thaliana leaves at 24 h post-inoculation. Both CT and CTr increased the accumulation of PATHOGENESIS-RELATED 1 (PR1) protein. However, CT and CTr had no significant effect on resistance to biotrophic pathogens in both compatible and incompatible plant-pathogen interactions. Nonetheless, our results revealed that CTr plays a critical role in increasing plant susceptibility to the necrotrophic pathogen ECC. Exogenous application of CT and CTr increased the induction of callose deposition in both the absence and presence of ECC. Increased callose deposition was detected in salicylic acid induction-deficient Arabidopsis mutant sid2 but to a lesser extent in ethylene signaling mutants, ein2-1 and etr1. Overall, our results suggest that ethylene signaling is related to CT and CTr-induced callose deposition and contributes to plant defense against pathogens, whereas salicylic acid is not required for this response.