Chk1-cyclin A/Cdk1 axis regulates origin firing programs in mammals
Chk1-cyclin A/Cdk1 axis regulates origin firing programs in mammals
复制标题
DOI：
10.1007/s10577-009-9086-2
复制
发表时间：
2010-01-01
影响因子：
2.6
通讯作者：
Shimada, Midori
中科院分区：
文献类型：
--
作者：
Nakanishi, Makoto;Katsuno, Yuko;Shimada, Midori
DNA 复制是确保有丝分裂前基因组 DNA 完全复制的关键，并且受到细胞周期机制和检查点信号的严格调控。 S 期程序的调节发生在几个阶段，影响起始激发、复制叉伸长、复制叉速度和复制叉稳定性，所有这些都依赖于 S 期促进激酶活性。体细胞哺乳动物细胞使用完善的起源程序，通过该程序在精确的时间复制基因组的特定区域。然而，S 期激酶调节哺乳动物起源放电的机制在很大程度上尚不清楚。在这里，我们讨论了在理解哺乳动物 S 期程序如何在正确的区域和适当的时间进行调节方面的最新进展。
DNA replication is key to ensuring the complete duplication of genomic DNA prior to mitosis and is tightly regulated by both cell cycle machinery and checkpoint signals. Regulation of the S phase program occurs at several stages, affecting origin firing, replication fork elongation, fork velocity, and fork stability, all of which are dependent on S-phase-promoting kinase activity. Somatic mammalian cells use well-established origin programs by which specific regions of the genome are replicated at precise times. However, the mechanisms by which S phase kinases regulate origin firing in mammals are largely unknown. Here, we discuss recent advances in the understanding of how S phase programs are regulated in mammals at the correct regions and at the appropriate times.