Electroacupuncture treatment ameliorated the long-term cognitive impairment via activating eNOS/NO pathway and related Aβ downregulation in sepsis-survivor mice.
Electroacupuncture treatment ameliorated the long-term cognitive impairment via activating eNOS/NO pathway and related Aβ downregulation in sepsis-survivor mice.
复制标题
电针治疗通过激活 eNOS/NO 途径和相关 Aβ 下调脓毒症幸存者小鼠改善长期认知障碍。
DOI：
10.1016/j.physbeh.2021.113646
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发表时间：
2022
影响因子：
2.9
通讯作者：
Song Jiangang
中科院分区：
文献类型：
--
作者：
Jun Guo;Yong Yue;Lu Liyue;Gao Hao;Yin Zhiyu;Wei Pan;Sun Long;Ruan Wenqing;Zou Yinghua;He He;Song Wei;Tong Qiuyu;Wang Xiongbiao;Wang Yongqiang;Song Jiangang
目的脓毒症是世界范围内重症监护病房面临的主要挑战，败血症幸存者留下了长期的认知缺陷。本研究旨在探讨电针对脓毒症存活小鼠长期认知功能的影响及其机制。方法采用盲肠结扎穿刺法建立C57BL/6雄性小鼠脓毒症模型。术后7天，电针或非穴位电针治疗脓毒症存活小鼠，连续17天，每日2次。用Morris水迷宫测试大鼠的认知功能。术后30d取小鼠海马区组织。结果与假手术对照组相比，脓毒症存活者存在明显的认知功能障碍，表现为逃避潜伏期延长，穿越次数减少，NO和p-eNOS水平降低，Aβ水平升高。结果与假手术对照组相比，脓毒症存活者存在明显的认知功能障碍。电针GV20、ST36穴位而非非穴位电针治疗可改善脓毒症存活小鼠的认知功能，提高NO和p-eNOS水平，减少A-β生成，eNOS抑制剂L可削弱电针治疗的效果。结论反复电针治疗可通过调控p-eNOS及相关Aβ的表达，改善脓毒症存活小鼠的长期认知功能障碍。
ObjectiveSepsis is a major challenge in intensive care unit worldwide and the septic survivors are left with long-term cognitive deficits. This work aims to explore the effects of electroacupuncture (EA) on long-term cognitive function and its underlying mechanism in sepsis-survivor mice.MethodsSepsis was induced by cecal ligation and puncture in C57BL/6 male mice. Seven days post-surgery, sepsis-survivor mice were treated with EA or nonacupoint EA for 17 days twice daily. Then, cognitive function was evaluated by Morris water maze task. The hippocampus tissue were collected from the mice at 30 days post-surgery. The level of nitric oxide and the expression of endothelial nitric oxide (eNOS), phospho-eNOS (p-eNOS), and amyloid β–peptide (Aβ) were measured.ResultsCompared with the sham-operated control, sepsis-survivors had significant cognitive deficits evidenced by the increased time of escape latency and reduced crossing number in Morris water maze task, as well as lower NO and p-eNOS level and higher Aβ level. EA treatment at GV20 and ST36 acupoints but not at a nonacupoint improved the cognitive function, increased the NO and p-eNOS level, and decreased Aβ generation; while eNOS inhibitor (l-NAME) undermined the efficacy of EA treatment.ConclusionIn conclusion, repeated EA treatment could ameliorate the long-term cognitive impairment via manipulating the expression of p-eNOS and related Aβ in sepsis-survivor mice.