Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation.
Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation.
复制标题
通过免疫沉淀检测 c-Myc 蛋白-蛋白相互作用和磷酸化状态。
DOI：
10.1007/978-1-62703-429-6_5
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Sears,RosalieC
中科院分区：
文献类型：
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作者：
Daniel,ColinJ;Zhang,Xiaoli;Sears,RosalieC
Co-immunoprecipitation is an invaluable technique in evaluating native protein–protein interactions in vitro and in vivo. However, it can be difficult to detect interactions of a very transient nature, particularly interactions with phosphatases and kinases. The evaluation of the phosphorylation status of c-Myc can also be challenging with the current commercially available phosphorylation sensitive antibodies. Here, we describe two protocols: one for the co-immunoprecipitation of endogenous c-Myc to detect protein–protein interactions and second, for the immunoprecipitation of endogenous c-Myc to probe for phosphorylation status.
