P-selectin glycoprotein ligand-1 is broadly expressed in cells of myeloid, lymphoid, and dendritic lineage and in some nonhematopoietic cells
P-selectin glycoprotein ligand-1 is broadly expressed in cells of myeloid, lymphoid, and dendritic lineage and in some nonhematopoietic cells
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DOI：
10.1182/blood.v88.8.3010.bloodjournal8883010
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发表时间：
1996-10-15
期刊：
影响因子：
20.3
通讯作者：
Moore, KL
中科院分区：
文献类型：
--
作者：
Laszik, Z;Jansen, PJ;Moore, KL
P-selectin glycoprotein ligand-1（PSGL-1）是一种粘蛋白样糖蛋白，可与骨髓细胞和淋巴细胞亚群上的P-和E-选择素配体结合。我们使用流式细胞术和免疫组化检查PSGL-1的表达对少数白细胞群体，分化造血细胞，和非造血组织使用两种单克隆抗体不同的蛋白表位PSGL-1。在骨髓中，PSGL-1在从成髓细胞阶段到分叶中性粒细胞的髓样细胞上表达，但在成红细胞或巨核细胞上未检测到。所有类型的循环髓样细胞表达PSGL-1，并且PSGL-1在髓样细胞外渗到组织中后被保留。PSGL-1还表达于循环树突状细胞、单核细胞来源的树突状细胞、淋巴组织和表皮中的树突状细胞以及滤泡树突状细胞上。检测的所有类型的淋巴细胞均表达PSGL-1，包括未成熟和成熟胸腺细胞、初始和记忆T细胞、γ/δ T细胞、自然杀伤细胞、B细胞和CD 34（+）祖细胞。然而，PSGL-1水平显着低于扁桃体淋巴细胞比循环淋巴细胞，表明PSGL-1的表达下调期间或之后进入次级淋巴组织的淋巴细胞。虽然PSGL-1抗原主要在造血细胞上检测到，但它也存在于输卵管上皮上。此外，PSGL-1抗原在一些病理组织中的微血管内皮上零星检测到。这表明PSGL-1可能具有介导白细胞粘附以外的功能。(C)1996年，美国血液学会。
P-selectin glycoprotein ligand-1 (PSGL-1) is a mucin-like glycoprotein ligand for P- and E-selectin on myeloid cells and a subset of lymphocytes. We used flow cytometry and immunohistochemistry to examine expression of PSGL-1 on minor leukocyte populations, differentiating hematopoietic cells, and nonhematopoietic tissues using two monoclonal antibodies to distinct protein epitopes on PSGL-1. In the bone marrow, PSGL-1 was expressed on myeloid cells from the myeloblast stage to the segmented neutrophil, but was not detected on erythroblasts or megakaryocytes. All types of circulating myeloid cells expressed PSGL-1, and PSGL-1 was retained after extravasation of myeloid cells into tissues. PSGL-1 was also expressed on circulating dendritic cells, monocyte-derived dendritic cells, dendritic cells in lymphoid tissues and epidermis, and follicular dendritic cells. All types of lymphoid cells examined expressed PSGL-1, including immature and mature thymocytes, naive and memory T cells, gamma/delta T cells, natural killer cells, B cells, and CD34(+) progenitor cells. However, PSGL-1 levels were substantially lower on tonsillar lymphocytes than on circulating lymphocytes, suggesting that PSGL-1 expression is downregulated during or after entry of lymphocytes into secondary lymphoid tissue. Although PSGL-1 antigen was detected primarily on hematopoietic cells, it was also present on the epithelium of the fallopian tube. Furthermore, PSGL-1 antigen was detected sporadically on microvascular endothelium in some pathologic tissues. This suggests that PSGL-1 may have functions other than mediating leukocyte adhesion. (C) 1996 by The American Society of Hematology.