Diagnosis of pancreatic cancer by K-ras point mutation and cytology of pancreatic juice.
Diagnosis of pancreatic cancer by K-ras point mutation and cytology of pancreatic juice.
复制标题
通过 K-ras 点突变和胰液细胞学诊断胰腺癌。
DOI：
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发表时间：
1996
影响因子：
9.8
通讯作者：
S. Ishiguro
中科院分区：
文献类型：
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作者：
H. Uehara;A. Nakaizumi;M. Baba;H. Iishi;M. Tatsuta;T. Kitamura;H. Ohigashi;O. Ishikawa;A. Takenaka;S. Ishiguro
目标
OBJECTIVES
Recently, it was reported that detection of K-ras point mutation at codon 12 in pancreatic juice is an objective method for the diagnosis of pancreatic cancer, but a few reports have suggested that this might represent an early event in pancreatic oncogenesis. In the present study we examined, in various patients, the occurrence of K-ras codon 12 point mutation in pancreatic juice and compared it with pancreatic juice cytology, which is also a reliable diagnostic method.
METHODS
Pancreatic juice was obtained endoscopically from patients with various pancreatic disorders and those without definite diseases, and was examined cytologically and for the occurrence of K-ras codon 12 point mutation. The K-ras gene was amplified by polymerase chain reaction (PCR) and the mutation at codon 12 (GGT-->GAT) was examined by slot blot hybridization analysis.
RESULTS
K-ras point mutation at codon 12 was detected in seven of 14 (50%) pancreatic cancers, in four of 10 (40%) mucin-producing tumors, in four of 13 (31%) chronic pancreatitis, and in two of 10 (20%) pancreas without definite disorders. K-ras point mutation was detected in nine of 18 (50%) pancreatic juice samples containing cancer cells, in eight of 18 (44%) pancreatic juice samples containing atypical cells, but in none of such samples containing only normal cells.
CONCLUSION
Cancer cells were detected from pancreatic cancer exclusively, but K-ras point mutation at codon 12 was detected in pancreatic juice, not only from pancreatic cancer, but also from other diseases.