In Vitro-Transcribed Chrysanthemum stunt viroid RNA Is Infectious to Chrysanthemum and Other Plants
In Vitro-Transcribed Chrysanthemum stunt viroid RNA Is Infectious to Chrysanthemum and Other Plants
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DOI：
10.1094/phyto-99-1-0058
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发表时间：
2009-01-01
期刊：
影响因子：
3.2
通讯作者：
Penmetcha, Kumar K. R.
中科院分区：
文献类型：
--
作者：
Matsushita, Yosuke;Penmetcha, Kumar K. R.
菊花矮化类病毒（Chrysanthemum stunt viroid，CSVd）是引起菊花矮化病的一种非编码RNA，严重影响菊花的产量。为了深入了解CSVd复制，感染，以及CSVd疾病在菊花植物中传播的原因，我们制备了线性CSVd RNA，并分析了其在菊花植物中引起疾病的能力。我们发现，线性CSVd复制有效的CSVd RNA从感染的菊花植物分离。此外，线性CSVd RNA的能力进行了评估，以及感染其他植物，这表明CSVd有一个广泛的寄主范围，其复制。重要的是，从这些宿主中分离的CSVd对菊花植物具有感染性，因此可能有助于疾病向菊花植物的传播。
Chrysanthemum stunt viroid (CSVd), a noncoding RNA, is known to cause chrysanthemum stunt disease, which affects the yield of flowers. To gain insights into CSVd replication, infection, and the reasons for the spreading of CSVd disease in chrysanthemum plants, we prepared linear CSVd RNA and analyzed its ability to cause disease in chrysanthemum plants. We found that linear CSVd replicated as efficiently as CSVd RNA isolated from the infected chrysanthemum plants. Additionally, the linear CSVd RNA was evaluated for its ability to infect other plants as well, which revealed that CSVd has a wide host range for its replication. Importantly, the CSVd isolated from these hosts is infectious to chrysanthemum plants, and thus potentially contributes to the spreading of the disease to chrysanthemum plants.