Shotgun lipidomics in substantiating lipid peroxidation in redox biology: Methods and applications.
Shotgun lipidomics in substantiating lipid peroxidation in redox biology: Methods and applications.
复制标题
鸟枪法脂质组学在氧化还原生物学中证实脂质过氧化：方法和应用
DOI：
10.1016/j.redox.2017.04.030
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发表时间：
2017-08
期刊：
影响因子：
11.4
通讯作者：
Han X
中科院分区：
文献类型：
--
作者：
Hu C;Wang M;Han X
基于多维质谱的鸟枪脂组学(MDMS-SL)在细胞脂质的综合分析方面取得了深刻的进展。它是直接从生物样品的脂类提取物中分析脂类的最强大的工具之一。它能够以高准确度/精密度分析近50种脂类和数千种单独的脂类。氧化还原失衡导致氧化应激，导致脂质过氧化，改变脂质代谢和动态平衡。氧化脂肪酸、4-羟基烯类和血浆蛋白原等类脂类对氧化应激反应敏感，或与氧化还原失衡相对应。因此，对这些脂类的准确评估不仅可以提供氧化还原状态，而且可以从分子水平深入了解疾病的发病机制。本文综述了MDMS-SL在分析这些脂类和分子物种方面的进展，并总结了它们在生物医学/生物研究中的最新代表性应用。我们相信，MDMS-SL可以通过证实氧化应激相关条件下异常的脂代谢、信号转导、转运和动态平衡，对氧化还原生物学做出重大贡献。脂质过氧化产物的鸟枪式脂肪组学分析。用鸟枪脂组学方法测定交替脂类。鸟枪式脂质组学揭示了可能的生化机制和生物标记物。用鸟枪脂组学评估系统性红斑狼疮的氧化还原生物学和神经毒性。
Multi-dimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) has made profound advances for comprehensive analysis of cellular lipids. It represents one of the most powerful tools in analyzing lipids directly from lipid extracts of biological samples. It enables the analysis of nearly 50 lipid classes and thousands of individual lipid species with high accuracy/precision. The redox imbalance causes oxidative stress, resulting in lipid peroxidation, and alterations in lipid metabolism and homeostasis. Some lipid classes such as oxidized fatty acids, 4-hydroxyalkenal species, and plasmalogen are sensitive to oxidative stress or generated corresponding to redox imbalance. Therefore, accurate assessment of these lipid classes can provide not only the redox states, but also molecular insights into the pathogenesis of diseases. This review focuses on the advances of MDMS-SL in analysis of these lipid classes and molecular species, and summarizes their recent representative applications in biomedical/biological research. We believe that MDMS-SL can make great contributions to redox biology through substantiating the aberrant lipid metabolism, signaling, trafficking, and homeostasis under oxidative stress-related condition. Shotgun lipidomic analysis of lipid peroxidation products. Determination of alternated lipid species by shotgun lipidomics. Shotgun lipidomics revealed possible biochemical mechanisms and biomarkers. Assessment of redox biology in SLE disease and neurotoxicity by shotgun lipidomics.