The Construction of Tissue-Engineered Blood Vessels Crosslinked with Adenosine-Loaded Chitosan/β-Cyclodextrin Nanoparticles using a Layer-by-Layer Assembly Method
The Construction of Tissue-Engineered Blood Vessels Crosslinked with Adenosine-Loaded Chitosan/β-Cyclodextrin Nanoparticles using a Layer-by-Layer Assembly Method
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DOI：
10.1002/adhm.201400167
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发表时间：
2014-11-01
影响因子：
10
通讯作者：
Zhu, Chuhong
中科院分区：
文献类型：
--
作者：
Chen, Wen;Zeng, Wen;Zhu, Chuhong
DOI: 10.1002/adhm. 201400167 enter the active state of proliferation and differentiation.[8] In addition, other studies have reported that adenosine can promote the mobilization of hematopoietic stem cells, but the mobilization mechanism is currently unclear.[9] The process of stem cell mobilization is essentially the process by which stationary bone marrow stem cells are mobilized into peripheral blood and are transformed into mobile stem cells, and this process requires externally provided energy. Adenosine is the end product of energy metabolism. Whether adenosine can promote EPC mobilization and whether the mobilization mechanism relies on the adenosine receptor pathway to stimulate the secretion of mobilization related factors or relies on the adenosine kinase pathway to provide energy for EPCs still require further investigation.Due to the important biological functions of adenosine, it has been used clinically as a drug. However, adenosine is quickly taken up by cells and has a very short half-life.[6] Scientists have used many methods to control the release of adenosine, including the construction of adenosine kinase-deficient embryonic stem (ES) cells and silk polymer-based adenosine-releasing materials.[10] After transplantation, TEBV have to face fluid shear stresses of high intensities and an extremely complex microenvironment. Therefore, there are high requirements for the stability and safety of biomaterials and the methods described above do not satisfy the requirements of the TEBV construction. Due to the special molecular structure, β-cyclodextrin (β-CD) can form inclusion complexes with most compounds, is prone to form stable hydrates, and can be used for the controlled release of drugs.[11] β-CD and chitosan can form stable nanoparticles via sodium tripolyphosphate (TPP) that are stable and are capable of the controlled release of drugs significantly more efficiently than pure chitosan nanoparticles.[12] On the basis of previous experiences with TEBV construction, we used a layer-by-layer assembly method to build TEBV using acellular vascular matrix, collagen and CS/β-CD nanoparticles loaded with adenosine.[4, 13] We investigated the function of adenosine in the rapid endothelialization and maintenance of the long-term patency of small-diameter TEBV as well as the underlying mechanism.
