Validation of green tea polyphenol biomarkers in a phase II human intervention trial.
Validation of green tea polyphenol biomarkers in a phase II human intervention trial.
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DOI：
10.1016/j.fct.2007.08.007
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
Jia-Sheng Wang;Haitao Luo;Piwen Wang;L. Tang;Jiahua Yu;Tianren Huang;S. Cox;Weimin Gao
中科院分区：
文献类型：
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作者：
Jia-Sheng Wang;Haitao Luo;Piwen Wang;L. Tang;Jiahua Yu;Tianren Huang;S. Cox;Weimin Gao
绿茶多酚(GTP)的健康益处在许多动物模型中都有报道，但人类研究尚未得出结论。部分原因是缺乏代表绿茶消费的生物标志物。在这项研究中，对124名接受500或1000毫克GTP或安慰剂3个月的健康成年人进行了第二阶段干预试验收集的血浆和尿样中GTP成分和代谢物的分析。在GTP干预后1个月和3个月，血浆(−)-表儿茶素-3-没食子酸酯(−)-3-没食子儿茶素没食子酸酯(P<；0.001，趋势检验)和(EGCG)-表儿茶素-3-没食子酸酯(P<；0.05，趋势检验)浓度均有显著的剂量依赖性升高。血浆(−)-表没食子儿茶素(EC)或(−)-表儿茶素(EGC)水平在干预后未见明显升高。混合效应模型显示剂量(EGCG)和逐时间剂量交互作用(ECG)有显著影响，但对EC和EGC没有显著影响。对2相代谢结合物的分析表明，血浆中的游离GTP占主导地位，EGCG高达85%，而尿中的大多数GTP是硫酸盐化和葡萄糖醛酸化的结合物(EC高达100%，EGC高达89%)。这些结果表明，血浆心电图和EGCG浓度是在人群水平上预测绿茶消费的可靠生物标志物。
Health benefits of green tea polyphenols (GTPs) have been reported in many animal models, but human studies are inconclusive. This is partly due to a lack of biomarkers representing green tea consumption. In this study, GTP components and metabolites were analyzed in plasma and urine samples collected from a phase II intervention trial carried out in 124 healthy adults who received 500- or 1000-mg GTPs or placebo for 3 months. A significant dose-dependent elevation was found for (−)-epicatechin-3-gallate (ECG) (p<0.001, trend test) and (−)-epigallocatechin-3-gallate (EGCG) (p<0.05, trend test) concentrations in plasma at both 1-month and 3-months after intervention with GTPs. No significant increase of (−)-epicatechin (EC) or (−)-epigallocatechin (EGC) was observed in plasma after GTP intervention. A mixed-effects model indicated significant effects of dose (EGCG) and dose by time interaction (ECG), but not for EC and EGC. Analysis of phase 2 metabolic conjugates revealed a predominance of free GTPs in plasma, up to 85% for EGCG, while a majority of GTPs in urine were sulfated and glucuronidated conjugates (up to 100% for EC and 89% for EGC). These results suggest that plasma ECG and EGCG concentrations are reliable biomarkers for green tea consumption at the population level.