Bio-capture of S. Typhimurium from surface water by aptamer for culture-free quantification.
Bio-capture of S. Typhimurium from surface water by aptamer for culture-free quantification.
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DOI：
10.1016/j.ecoenv.2011.11.039
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发表时间：
2012-04
影响因子：
6.8
通讯作者：
Gulshan Singh;P. Vajpayee;Neetika Rani;Anurag Jyoti;K. Gupta;R. Shanker
中科院分区：
文献类型：
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作者：
Gulshan Singh;P. Vajpayee;Neetika Rani;Anurag Jyoti;K. Gupta;R. Shanker
在这项研究中，一种DNA适配体被用于生物捕获从地表水收集的高度流行区的肠道沙门氏菌血清型鼠伤寒沙门氏菌，然后通过基于分子信标的实时PCR检测靶向invA基因的无培养检测。该方法可检出S.鼠伤寒沙门氏菌细胞（1CFU/PCR或100CFU/ml）被血清型特异性DNA适体选择性捕获。结果表明，Gomti河水样（n=40）均受到S.鼠伤寒沙门氏菌（31400-1× 107 CFU/100 ml）。发现基于血清型特异性DNA适体的细菌细胞生物捕获形式的预分析步骤在检测S.显示天然PCR抑制剂和高背景细菌植物群的环境样本中的鼠伤寒。该检测方法可用于南亚地区地表沃茨的定期监测，以预测和管理非伤寒沙门氏菌病。
In this study, a DNA aptamer was used to bio-capture Salmonella enterica serovar Typhimurium from surface water collected from highly endemic zone prior to culture-free detection through Molecular-Beacon based real-time PCR assay targeting invA gene. The assay could detect S. Typhimurium cells (1CFU/PCR or 100CFU/ml) selectively captured by serovar specific DNA aptamer. The observations indicate that all the water samples (n=40) collected from the river Gomti were contaminated by S. Typhimurium (31400–1×107CFU/100ml). The pre-analytical step in the form of serovar specific DNA aptamer based bio-capture of the bacterial cell was found to enhance the sensitivity of the florescent probe based real-time PCR assay during detection of S. Typhimurium in environmental samples exhibiting natural PCR inhibitors and high background bacterial flora. The assay could be used for the regular monitoring of surface waters for forecasting and management of non-typhoidal Salmonellosis in south Asia.