Pma1, a P-type Proton ATPase, Is a Determinant of Chronological Life Span in Fission Yeast
Pma1, a P-type Proton ATPase, Is a Determinant of Chronological Life Span in Fission Yeast
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DOI：
10.1074/jbc.m110.175562
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发表时间：
2010-11-05
影响因子：
4.8
通讯作者：
Aiba, Hirofumi
中科院分区：
文献类型：
--
作者：
Ito, Hirokazu;Oshiro, Tomoko;Aiba, Hirofumi
按时间顺序的寿命是由细胞在非分裂状态下能存活多久来定义的。在酵母中，通过进入稳定期后的活力来测量。迄今为止，已经确定了一些影响实足寿命的因素，然而，这些因素如何调节实足寿命的分子细节尚未阐明清楚。由于寿命是一个复杂的现象，被认为是由许多因素调节，因此有必要确定影响时间寿命的新因素，以了解寿命调节。为此，我们筛选了长寿的突变体，并确定Pma1，一个必不可少的P型质子ATP酶，作为时间寿命的决定因素之一。我们发现，部分损失的Pma1活性不仅突变，而且通过治疗与Pma1抑制性化学钒酸盐导致在粟酒裂殖酵母的长寿表型。这些发现提出了一种通过调节Pma1作为分子靶点来操纵时序寿命的新方法。
Chronological life span is defined by how long a cell can survive in a non-dividing state. In yeast, it is measured by viability after entry into stationary phase. To date, some factors affecting chronological life span have been identified; however, the molecular details of how these factors regulate chronological life span have not yet been elucidated clearly. Because life span is a complicated phenomenon and is supposedly regulated by many factors, it is necessary to identify new factors affecting chronological life span to understand life span regulation. To this end, we have screened for long-lived mutants and identified Pma1, an essential P-type proton ATPase, as one of the determinants of chronological life span. We show that partial loss of Pma1 activity not only by mutations but also by treatment with the Pma1 inhibitory chemical vanadate resulted in the long-lived phenotype in Schizosaccharomyces pombe. These findings suggest a novel way to manipulate chronological life span by modulating Pma1 as a molecular target.