RELATIONSHIP BETWEEN MALONDIALDEHYDE PRODUCTION AND ARACHIDONATE CONSUMPTION DURING NADPH-SUPPORTED MICROSOMAL LIPID-PEROXIDATION
RELATIONSHIP BETWEEN MALONDIALDEHYDE PRODUCTION AND ARACHIDONATE CONSUMPTION DURING NADPH-SUPPORTED MICROSOMAL LIPID-PEROXIDATION
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DOI：
10.1016/0006-2952(82)90034-x
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发表时间：
1982-01-01
影响因子：
5.8
通讯作者：
SCHENKMAN, JB
中科院分区：
文献类型：
--
作者：
JORDAN, RA;SCHENKMAN, JB
在大鼠、小鼠、豚鼠和兔子的肝微粒体中，在 NADPH 支持的脂质过氧化之前和期间测定了脂肪酸浓度和丙二醛 (MDA) 形成。与之前的报道一致，大鼠的 MDA 产量最高，其次是小鼠，豚鼠和兔子的产量要少得多。大鼠和小鼠的总不饱和脂肪酸（18:1、18:2、18:3、20:3、20:4、20:5、22:5、22:6）的微粒体含量大致相同，而豚鼠和兔子的含量较低。对于所有物种来说，脂质过氧化都会导致多不饱和脂肪酸随时间的减少，特别是 20:4 和 22:6。这些下降对于大鼠和小鼠来说最为明显。正如其他人报道的那样，大鼠饮食方案的改变导致微粒体脂肪酸含量发生显着变化，并且还导致脂质过氧化过程中 MDA 产生率和多不饱和脂肪酸消耗率发生变化。对每种动物物种和饲喂不同饮食的大鼠的 MDA 产生率和个体不饱和脂肪酸消耗率进行了比较。在单个微粒体制剂和不同物种中，MDA 产生与 20:4 消失之间存在线性关系。 20:4 的初始微粒体浓度和 MDA 形成的初始速率也存在类似的关系。其他不饱和脂肪酸不表现出线性关系。测量了不同物种的各种微粒体混合功能氧化酶变量。没有发现这些值与 MDA 产量之间有直接关系。
Fatty acid concentrations and malondialdehyde (MDA) formation were determined before and during NADPH-supported lipid peroxidation in liver microsomes from rat, mouse, guinea pig and rabbit. In agreement with earlier reports, MDA production was greatest for rat, followed by mouse, and much less for guinea pigs and rabbit. The microsomal content of total unsaturated fatty acids (18:1, 18:2, 18:3, 20:3, 20:4, 20:5, 22:5, 22:6) was approximately the same for rat and mouse and was lower in guinea pig and rabbit. Lipid peroxidation caused a time-dependent decrease in the polyunsaturated fatty acids, particularly 20:4 and 22:6, for all species. These decreases were most pronounced for rat and mouse. Alterations in the dietary regime for rat produced marked changes in microsomal fatty acid content as reported by others, and also caused changes in the rates of MDA production and polyunsaturated fatty acid consumption during lipid peroxidation. A comparison between the rates of MDA production and the rates of individual unsaturated fatty acid consumption was performed for each animal species and for rats fed different diets. A linear relatonship was found between MDA production and 20:4 disappearance in individual microsomal preparations and in different species. A similar relationship was seen for the initial microsomal concentration of 20:4 and the initial rate of MDA formation. Other unsaturated fatty acids did not exhibit linear relationships. Various microsomal mixed-function oxidase variables were measured for the different species. No direct relationship between these values and MDA production was found.