Applications of tandem mass spectrometry to the characterization of derivatized glutathione conjugates. Studies with S-(N-methylcarbamoyl)glutathione, a metabolite of the antineoplastic agent N-methylformamide.
Applications of tandem mass spectrometry to the characterization of derivatized glutathione conjugates. Studies with S-(N-methylcarbamoyl)glutathione, a metabolite of the antineoplastic agent N-methylformamide.
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串联质谱法在衍生化谷胱甘肽缀合物表征中的应用。
DOI：
10.1002/bms.1200160110
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Baillie,TA
中科院分区：
文献类型：
--
作者：
Pearson,PG;Threadgill,MD;Howald,WN;Baillie,TA
报道了 S-(N-甲基氨基甲酰基)谷胱甘肽 (1) 及其一系列烷氧基羰基甲酯衍生物的快原子轰击质谱法产生的 [M + H]+ 离子的子离子光谱。在 1 及其乙氧基和苄氧基羰基甲酯的碰撞激活解离 (CAD) 光谱中观察到结构信息丰富的偶数电子碎片离子，这些离子用于定义缀合物的异生物质和肽成分的性质。另一方面，对 tBOC 甲酯衍生物的研究表明，tBOC 基团对 CAD 过程具有强大的直接影响，并且这种情况下的主要子离子与 tBOC 功能本身的裂解有关，诊断价值不大。在所检查的衍生物中，从易于形成、理想的高性能液相色谱特性以及 CAD 条件下信息丰富的质谱碎片特征的角度来看，在水介质中很容易由 1 生成的苄氧基羰基同系物被认为是最有用的。
Daughter ion spectra are reported for [M + H]+ions generated by fast atom bombardment mass spectrometry ofS‐(N‐methylcarbamoyl)glutathione (1) and a series of alkoxycarbonyl methyl ester derivatives thereof. Structurally informative, even‐electron fragment ions, which serve to define the nature of both the xenobiotic and peptide components of the conjugate, are observed in the collisionally activated dissociation (CAD) spectra of 1 and its ethoxy‐ and benzyloxycarbonyl methyl esters. Studies with thet‐butyloxycarbonyl (tBOC) methyl ester derivative, on the other hand, indicated that thetBOC group exerts a powerful directing influence on the CAD process, and that the major daughter ions in this case are associated with cleavage of thetBOC functionality itself and are of little diagnostic value. Of the derivatives examined, the benzyloxycarbonyl congener, which may be generated readily from 1 in aqueous media, is judged to be the most useful from the standpoints of ease of formation, desirable high‐performance liquid chromatographic properties, and informative mass spectral fragmentation characteristics under CAD conditions.