Cationic Liposomes: A Flexible Vaccine Delivery System for Physicochemically Diverse Antigenic Peptides.
Cationic Liposomes: A Flexible Vaccine Delivery System for Physicochemically Diverse Antigenic Peptides.
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DOI：
10.1007/s11095-018-2490-6
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发表时间：
2018-09-12
影响因子：
3.7
通讯作者：
Jiskoot W
中科院分区：
文献类型：
--
作者：
Heuts J;Varypataki EM;van der Maaden K;Romeijn S;Drijfhout JW;van Scheltinga AT;Ossendorp F;Jiskoot W
个性化的基于肽的癌症疫苗将由多种患者特异性合成长肽（SLP）组成，其可能具有各种理化性质。为了配制这样的SLP，需要灵活的疫苗递送系统。我们研究了阳离子脂质体是否适用于此目的。通过脱水-再水化方法，将15个含有SIINFEKL T细胞表位的SLP（疏水性和等电点差异很大）分别装载在阳离子脂质体中。通过动态光散射（DLS）测量颗粒尺寸和多分散指数（PDI），并且通过激光多普勒电泳测量zeta电位。荧光测定肽负载，并在体外树突状细胞（DC）和CD 8 + T细胞的共培养物中评估配制的肽的免疫原性。根据SLP特征，通过使用三种不同的加载方法变体将所有SLP加载到阳离子脂质体中。十五种脂质体制剂具有相当的尺寸（< 200 nm）、PDI（< 0.3）和ζ电位（22-30 mV）。阳离子脂质体有效地将SLP递送至DC，随后活化SIINFEKL特异性CD 8 + T细胞，表明SLP的免疫活性改善。阳离子脂质体可以容纳各种不同的SLP，因此是个性化癌症疫苗的潜在递送平台。本文的在线版本（10.1007/s11095-018-2490-6）包含补充材料，可供授权用户使用。
Personalized peptide-based cancer vaccines will be composed of multiple patient specific synthetic long peptides (SLPs) which may have various physicochemical properties. To formulate such SLPs, a flexible vaccine delivery system is required. We studied whether cationic liposomes are suitable for this purpose. Fifteen SIINFEKL T cell epitope-containing SLPs, widely differing in hydrophobicity and isoelectric point, were separately loaded in cationic liposomes via the dehydration-rehydration method. Particle size and polydispersity index (PDI) were measured via dynamic light scattering (DLS), and zeta potential with laser Doppler electrophoresis. Peptide loading was fluorescently determined and the immunogenicity of the formulated peptides was assessed in co-cultures of dendritic cells (DCs) and CD8+ T-cells in vitro. All SLPs were loaded in cationic liposomes by using three different loading method variants, depending on the SLP characteristics. The fifteen liposomal formulations had a comparable size (< 200 nm), PDI (< 0.3) and zeta potential (22–30 mV). Cationic liposomes efficiently delivered the SLPs to DCs that subsequently activated SIINFEKL-specific CD8+ T-cells, indicating improved immunological activity of the SLPs. Cationic liposomes can accommodate a wide range of different SLPs and are therefore a potential delivery platform for personalized cancer vaccines. The online version of this article (10.1007/s11095-018-2490-6) contains supplementary material, which is available to authorized users.
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影响因子：
3.4
作者：
Campbell, RB;Balasubramanian, SV;Straubinger, RM
通讯作者：
Straubinger, RM
影响因子：
6.7
作者：
Ma, Yifan;Zhuang, Yan;Cai, Lintao
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影响因子：
4.4
作者：
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通讯作者：
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影响因子：
10.1
作者：
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影响因子：
4.5
作者：
Magdeldin S;Yoshida Y;Li H;Maeda Y;Yokoyama M;Enany S;Zhang Y;Xu B;Fujinaka H;Yaoita E;Sasaki S;Yamamoto T
通讯作者：
Yamamoto T