Adhesion of the entomopathogenic fungus Beauveria (Cordyceps) bassiana to substrata
Adhesion of the entomopathogenic fungus Beauveria (Cordyceps) bassiana to substrata
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DOI：
10.1128/aem.71.9.5260-5266.2005
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发表时间：
2005-09-01
影响因子：
4.4
通讯作者：
Keyhani, NO
中科院分区：
文献类型：
--
作者：
Holder, DJ;Keyhani, NO
昆虫病原真菌球孢白僵菌产生至少三种不同的单细胞繁殖体，气生分生孢子，营养细胞称为芽生孢子，和浸没分生孢子，其可以分别从琼脂平板，从丰富的肉汤液体培养物，和在营养限制条件下在浸没培养物中分离。荧光标记的真菌细胞用于定量这些细胞类型与具有各种疏水或亲水性质的表面的粘附动力学。气生分生孢子粘附弱极性表面和迅速的疏水性和亲水性表面，但可以很容易地洗掉后者的表面。与此相反，芽生孢子结合差的疏水表面，形成小的聚集体，迅速结合到亲水表面，并需要更长的孵育时间结合到弱极性表面比亲水表面。淹没分生孢子显示最广泛的结合特异性，坚持疏水性，弱极性和亲水性表面。B的附着力。在对糖苷酶和蛋白酶处理、pH和添加各种碳水化合物竞争剂和去污剂的敏感性方面，球孢菌细胞类型也不同。气生分生孢子的外细胞壁层含有十二烷基硫酸钠不溶性，三氟乙酸可溶性蛋白质（推测疏水蛋白），不存在于芽生孢子或浸没分生孢子。细胞表面性质的变化导致B的不同粘附质量。球孢的气生分生孢子、芽生孢子和沉水分生孢子的研究可以导致合理的设计决策，以提高昆虫病原真菌对宿主靶标的功效和可能的特异性。
The entomopathogenic fungus Beauveria bassiana produces at least three distinct single-cell propagules, aerial conidia, vegetative cells termed blastospores, and submerged conidia, which can be isolated from agar plates, from rich broth liquid cultures, and under nutrient limitation conditions in submerged cultures, respectively. Fluorescently labeled fungal cells were used to quantify the kinetics of adhesion of these cell types to surfaces having various hydrophobic or hydrophilic properties. Aerial conidia adhered poorly to weakly polar surfaces and rapidly to both hydrophobic and hydrophilic surfaces but could be readily washed off the latter surfaces. In contrast, blastospores bound poorly to hydrophobic surfaces, forming small aggregates, bound rapidly to hydrophilic surfaces, and required a longer incubation time to bind to weakly polar surfaces than to hydrophilic surfaces. Submerged conidia displayed the broadest binding specificity, adhering to hydrophobic, weakly polar, and hydrophilic surfaces. The adhesion of the B. bassiana cell types also differed in sensitivity to glycosidase and protease treatments, pH, and addition of various carbohydrate competitors and detergents. The outer cell wall layer of aerial conidia contained sodium dodecyl sulfate-insoluble, trifluoroacetic acid-soluble proteins (presumably hydrophobins) that were not present on either blastospores or submerged conidia. The variations in the cell surface properties leading to the different adhesion qualities of B. bassiana aerial conidia, blastospores, and submerged conidia could lead to rational design decisions for improving the efficacy and possibly the specificity of entomopathogenic fungi for host targets.