Studies on the inhibition of repair of ultraviolet- and methyl methanesulfonate-induced damage in the DNA of human fibroblasts by novobiocin.
Studies on the inhibition of repair of ultraviolet- and methyl methanesulfonate-induced damage in the DNA of human fibroblasts by novobiocin.
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新生霉素抑制紫外线和甲磺酸甲酯诱导的人成纤维细胞 DNA 损伤修复的研究。
DOI：
10.1093/nar/10.19.6207
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发表时间：
1982
影响因子：
14.9
通讯作者：
Regan,JD
中科院分区：
文献类型：
--
作者：
Snyder,RD;VanHouten,B;Regan,JD
抗生素新生物素被证明可以改变人细胞DNA在碱性蔗糖中的沉降特性。这种改变至少部分是由于新生物素存在时细胞中dna -蛋白结合增加。嘧啶二聚体分析和修复复制研究支持先前的报道，即新生物素抑制人体细胞中紫外线损伤的修复，但我们发现这种阻断是短暂的。CsCl密度离心和ara-C抑制技术也表明，新生物素对甲磺酸诱导的“长补丁”修复无效。然而，在新生物素存在的情况下，hms处理的细胞DNA中断裂的积累表明，一些“短补丁”烷基化修复可能被抗生素抑制。这些发现是根据新生物素可能抑制人类和细菌细胞中DNA旋回样活性的建议进行讨论的。
The antibiotic novobiocin is shown to alter the sedimentation properties of human cellular DNA in alkaline sucrose. This alteration is at least partially due to increased DNA-protein binding in the cell in the presence of novobiocin. Pyrimidine dimer analysis and repair replication studies support previous reports that novobiocin inhibits repair of UV damage in human cells but we find this block to be shortlived. It is also shown that novobiocin is ineffective at blocking “long-patch” repair induced by methyl methanesulfonate as measured both by CsCl density centrifugation and the ara-C inhibition technique. However, the accumulation of breaks in HMS-treated cellular DNA in the presence of novobiocin suggests that some “short-patch” alkylation repair may be inhibited by the antibiotic. These findings are discussed in light of the proposal that novobiocin may inhibit a DNA gyrase-like activity in human as in bacterial cells.