DNA conformational equilibrium enables continuous changing of curvatures
DNA conformational equilibrium enables continuous changing of curvatures
复制标题
DNA构象平衡使曲率不断变化
DOI：
10.1039/d2nr05404c
复制
发表时间：
2023
期刊：
影响因子：
6.7
通讯作者：
Mao, Chengde
中科院分区：
文献类型：
--
作者：
Mao, Dake;Paluzzi, Victoria E.;Zhang, Cuizheng;Mao, Chengde
用一小块瓷砖组装复杂的结构是生物学中的一个常见主题。例如，许多相同蛋白质的拷贝组成了多面体形状的病毒衣壳，而微管蛋白可以形成长的微管。这启发了基于瓷砖的DNA自组装的发展，用于纳米结构，特别是对于高对称性的结构。在最终的结构中，每种类型的母题都将采用相同的构象，要么是刚性的，要么是定义的柔性的。对于没有对称性的结构，它们的组装仍然是一小组瓷砖的挑战。为了迎接这一挑战，在计算科学的推动下，人们探索了算法自组装，但如何将这种方法实现到一维(1D)结构还不清楚。在这里，我们已经证明了构象平衡的不断变化可以允许一维结构的演化。原子力显微镜成像显示，一种类型的DNA瓷砖成功地组装成DNA螺旋和同心圆，从结构的中心向外变得越来越少弯曲。这项工作为基于瓷砖的DNA组装指明了一个新的方向。
Assembly of complex structures from a small set of tiles is a common theme in biology. For example, many copies of identical proteins make up polyhedron-shaped, viral capsids and tubulin can make long microtubules. This inspired the development of tile-based DNA self-assembly for nanoconstruction, particularly for structures with high symmetries. In the final structure, each type of motif will adopt the same conformation, either rigid or with defined flexibility. For structures that have no symmetry, their assembly remains a challenge from a small set of tiles. To meet this challenge, algorithmic self-assembly has been explored driven by computational science, but it is not clear how to implement this approach to one-dimensional (1D) structures. Here, we have demonstrated that a constant shift of a conformational equilibrium could allow 1D structures to evolve. As shown by atomic force microscopy imaging, one type of DNA tile successfully assembled into DNA spirals and concentric circles, which became less and less curved from the structure's center outward. This work points to a new direction for tile-based DNA assembly.