Concentration gradient generator using a convective-diffusive balance
Concentration gradient generator using a convective-diffusive balance
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DOI：
10.1039/b800859k
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发表时间：
2008-01-01
期刊：
影响因子：
6.1
通讯作者：
Lee, Ki Sung
中科院分区：
文献类型：
--
作者：
Kang, Taekyu;Han, Jeahyeong;Lee, Ki Sung
由于生物分子浓度梯度是各种生物现象的重要因素，为了建立稳定的体外生物分子浓度梯度，人们设计了许多利用微流体技术的浓度梯度发生器，每种浓度梯度发生器都有自己的优势。然而，所有的基本问题，设备的简单性，动态可控性和无对流条件，并没有同时得到解决。在本文中，我们描述了一种在逆流配置中使用对流-扩散平衡建立浓度梯度的新方法。该方法可在1分钟内形成稳定的、可重复的浓度梯度曲线，且曲线的形状易于用简单的数学公式预测。
Since biomolecular concentration gradients are an important factor in various biological phenomena, many concentration gradient generators utilizing microfluidic techniques have been devised in order to establish stable in vitro biomolecular concentration gradients, each with their own strengths. However, all the fundamental issues, simplicity in apparatus, dynamic controllability, and convection-free conditions, have not been addressed simultaneously. In this paper, we describe a novel way of establishing concentration gradients using a convective-diffusive balance in a counterflow configuration. With the help of this method, stable, reproducible concentration gradient profiles can be formed in under a minute and the shape of the profiles are easily predicted with a simple mathematical formula.