Regional expression of P2Y4 receptors in the rat central nervous system
Regional expression of P2Y4 receptors in the rat central nervous system
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DOI：
10.1007/s11302-011-9246-7
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发表时间：
2011-12-01
影响因子：
3.5
通讯作者：
Burnstock, Geoffrey
中科院分区：
文献类型：
--
作者：
Song, Xianmin;Guo, Wei;Burnstock, Geoffrey
P2 Y受体是由八个已知亚基（P2 Y（1，2，4，6，11，12，13，14））组成的G蛋白偶联受体，它们在神经组织中参与不同的功能。本研究应用免疫组织化学和原位杂交技术研究了P2 Y（4）受体在大鼠中枢神经系统（CNS）中的表达模式。免疫组化染色的特异性已通过预吸收，Western印迹，并结合使用免疫组化和原位杂交进行了验证。表达P2 Y（4）受体的神经元广泛分布于大鼠中枢神经系统。P2 Y（4）受体在下丘脑、红核、脑桥核、三叉神经中脑核、三叉神经运动核、疑核、下橄榄核、舌下神经核和迷走运动背核的大细胞神经内分泌神经元中有较强的免疫染色。神经元和星形胶质细胞均表达P2 Y（4）受体。P2 Y（4）受体免疫染色信号主要局限于神经元胞体和树突，提示P2 Y（4）受体主要参与调节突触后事件。在下丘脑，所有的加压素（VP）和催产素（OT）神经元和所有的食欲素A神经元均为P2 Y（4）受体免疫反应阳性。所有表达P2 Y（4）受体的神经元均表达N-甲基-d-天冬氨酸受体1（NR 1）。提示嘌呤和嘧啶类化合物可能通过P_2Y（4）受体参与调节下丘脑VP、OT和食欲素等神经肽的释放。此外，神经元的生理和病理生理功能可以通过P2 Y（4）受体和NR 1之间的偶联来起作用。
P2Y receptors are G protein-coupled receptors composed of eight known subunits (P2Y(1, 2, 4, 6, 11, 12, 13, 14)), which are involved in different functions in neural tissue. The present study investigates the expression pattern of P2Y(4) receptors in the rat central nervous system (CNS) using immunohistochemistry and in situ hybridization. The specificity of the immunostaining has been verified by preabsorption, Western blot, and combined use of immunohistochemistry and in situ hybridization. Neurons expressing P2Y(4) receptors were distributed widely in the rat CNS. Heavy P2Y(4) receptor immunostaining was observed in the magnocellular neuroendocrine neurons of the hypothalamus, red nucleus, pontine nuclei, mesencephalic trigeminal nucleus, motor trigeminal nucleus, ambiguous nucleus, inferior olive, hypoglossal nucleus, and dorsal motor vagus nucleus. Both neurons and astrocytes express P2Y(4) receptors. P2Y(4) receptor immunostaining signals were mainly confined to cell bodies and dendrites of neurons, suggesting that P2Y(4) receptors are mainly involved in regulating postsynaptic events. In the hypothalamus, all the vasopressin (VP) and oxytocin (OT) neurons and all the orexin A neurons were immunoreactive for P2Y(4) receptors. All the neurons expressing P2Y(4) receptors were found to express N-methyl-d-aspartate receptor 1 (NR1). These data suggest that purines and pyrimidines might be involved in regulation of the release of the neuropeptides VP, OT, and orexin in the rat hypothalamus via P2Y(4) receptors. Further, the physiological and pathophysiological functions of the neurons may operate through coupling between P2Y(4) receptors and NR1.