Relaxin's Involvement in Extracellular Matrix Homeostasis Two Diverse Lines of Evidence
Relaxin's Involvement in Extracellular Matrix Homeostasis Two Diverse Lines of Evidence
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DOI：
10.1111/j.1749-6632.2008.03801.x
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Konieczko, Elisa M.
中科院分区：
文献类型：
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作者：
Cooney, Timothy E.;Schober, Justine M.;Konieczko, Elisa M.
越来越多的证据表明，松弛素调节不同组织细胞外基质中的胶原蛋白。在不同的研究中，我们进一步证实了这一假设。免疫荧光法用于探测来自掌斜韧带外植体培养的分离的成纤维细胞的波形蛋白、肌动蛋白、RXFP 1和雌激素受体β。韧带作为手术废物从拇指重建患者中获得。迄今为止，已对四个标本进行了检查。来自这些患者的细胞表达波形蛋白和肌动蛋白，与成纤维细胞形态一致。假定的成纤维细胞来自三名女性患者中的两名表达RXFP 1受体;孤独的男性是阴性的。然而，由于样本较小，这些数据被认为是初步的。免疫组化被用于冷冻切片从26个皮肤活检获得的儿童接受生殖器成形术。还对一部分样本进行了转化生长因子（TGF-β 1）和TGF-β 3检测。使用了适当的对照。最后，通过使用基于酶联免疫吸附测定的方法对患者血液的子集进行松弛素测定。结果显示，在96%的患者中，RXFP 1受体在基底膜细胞中表达，无论性别如何。大多数组织表达TGF-β。最后，血清学表明，松弛素是检测这些儿童。我们的两条研究线为松弛素的不同组织向性提供了额外的证据。特别是，结缔组织如韧带和基底层角质形成细胞表达RXFP 1。这些数据支持我们的论点，松弛素影响韧带的完整性和伤口愈合。
Burgeoning evidence suggests that the hormone relaxin modulates collagen in the extracellular matrix of diverse tissues. In separate lines of study, we provide further substantiation of this hypothesis. Immunofluorescence was used to probe isolated fibroblasts derived from volar oblique ligament explant culture for vimentin, actin, RXFP1, and estrogen receptor beta. Ligaments were obtained as surgical waste from thumb reconstruction patients. Four specimens have been examined to date. Cells derived from these patients expressed vimentin and actin, consistent with fibroblast morphology. Putative fibroblasts derived from two of three female patients expressed RXFP1 receptors; the solitary male was negative. Given the small sample, however, the data are considered preliminary. Immunohistochemistry was used on frozen sections from 26 skin biopsies obtained from children undergoing genitoplasty. A subset of samples was also probed for transforming growth factor (TGF-beta 1) and TGF-beta 3. Appropriate controls were used. Finally, a subset of patient blood was assayed for relaxin by using an enzyme-linked immunosorbent assay-based method. The results showed RXFP1 receptor expression in the cells that populate the basement membrane in 96% of patients, regardless of gender. Most tissue expressed TGF-beta. Finally, serology suggested that relaxin was detectable in these children. Our two lines of research provide additional evidence for the diverse tissue tropism of relaxin. In particular, connective tissues as diverse as ligaments and basal lamina keratinocytes express RXFP1. These data lend support to our contention that relaxin affects ligament integrity and wound healing.