In vivo evidence for progesterone dependent decreases in serotonin release the hypothalamus and midbrain central grey: Relation to the induction of lordosis
In vivo evidence for progesterone dependent decreases in serotonin release the hypothalamus and midbrain central grey: Relation to the induction of lordosis
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DOI：
10.1016/0006-8993(95)01403-9
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发表时间：
1996-03-04
期刊：
影响因子：
2.9
通讯作者：
Renner, KJ
中科院分区：
文献类型：
--
作者：
Farmer, CJ;Isakson, TR;Renner, KJ
应用在体微透析技术研究了孕酮（P）对下丘脑腹内侧区（VMH）、视前区（POA）和中脑中央灰质（MCG）5-羟色胺（5-HT）溢出的影响。用水合氯醛麻醉用5 μ g雌二醇预处理的卵巢切除大鼠，并立体定向地植入指向相应脑部位之一的透析探针。细胞外5 HT水平稳定3至5小时后探针植入。在稳定的基线条件下，通过透析探针灌注1 μ M河豚毒素导致研究的脑区域中5 HT溢出减少50-65%。在测试P对5 HT溢出的影响的实验中，大鼠皮下注射0.5mg P或丙二醇载体。处理后，以20 min间隔分析样品的5 HT，持续4 h。灌流液中的5-HT水平没有显着变化的VMH，POA或MCG在溶剂处理的大鼠。同样，P处理未能显著改变POA中的5 HT溢出。在VMH，灌流液中的5-HT水平显着降低P治疗后60分钟。在MCG中，在P后20分钟检测到灌注液5 HT水平的降低。P处理后，在VMH和MCG中测量的5 HT溢出减少持续到采样期的剩余时间，VMH中的1个时间点除外。结果提供了在体内的证据P影响的VMH和MCG中的5 HT释放的减少。在MCG细胞外5 HT的快速减少表明，这种效果可能代表了P的非基因组作用。这些结果进行了讨论，在脊柱前凸行为的调节中的作用的5 HT。
The effects of progesterone (P) on serotonin (5HT) overflow in the ventromedial hypothalamus (VMH), preoptic area (POA) and midbrain central grey (MCG) were studied using in vivo microdialysis. Ovariectomized rats, pretreated with 5 mu g estradiol, were anesthetized with chloral hydrate and stereotaxically implanted with dialysis probes directed towards one of the respective brain sites. Extracellular 5HT levels stabilized 3 to 5 h following probe implantation. Under stable baseline conditions, perfusion of 1 mu M tetrodotoxin through the dialysis probe resulted in a 50-65% reduction in 5HT overflow in the brain areas studied. In experiments testing the effect of P on 5HT overflow, rats were subcutaneously injected with 0.5 mg P or propylene glycol vehicle. Samples were analyzed for 5HT at 20 min intervals for 4 h after treatment. Perfusate levels of 5HT were not significantly changed in the VMH, POA or MCG in vehicle-treated rats. Similarly, P treatment failed to significantly alter 5HT overflow in the POA. In the VMH, perfusate levels of 5HT were significantly reduced 60 min after P treatment. Decreases in perfusate 5HT levels were detected 20 min after P in the MCG. The decreases in 5HT overflow measured in the VMH and MCG following P treatment persisted for the remainder of the sampling period with the exception of 1 time point in the VMH. The results provide in vivo evidence for P-influenced decreases in 5HT release in the VMH and MCG. The rapid decrease in extracellular 5HT in the MCG suggests that this effect may represent a non-genomic action of P. These results are discussed in relation to the role of 5HT in the regulation of lordosis behavior.