Isolation and characterization of polymorphic microsatellite markers from Japanese grenadier anchovy (Coilia nasus)
Isolation and characterization of polymorphic microsatellite markers from Japanese grenadier anchovy (Coilia nasus)
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DOI：
10.1007/s12686-011-9532-2
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发表时间：
2012-06
影响因子：
1.1
通讯作者：
Jin-Quan Yang;Xiao-du Zhou;Dong Liu;Zhi-zhi Liu;Liang-jiang Wang;Wenqiao Tang
中科院分区：
文献类型：
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作者：
Jin-Quan Yang;Xiao-du Zhou;Dong Liu;Zhi-zhi Liu;Liang-jiang Wang;Wenqiao Tang
本研究利用PCR微卫星阵列技术分离并鉴定了12个新的多态性微卫星位点。C.用24个PCR引物对日本有明湾的日本产的日本每个位点的平均等位基因数为11.08（8 ~ 20）。观察到的杂合度值范围为0.562至1.0，预期杂合度值范围为0.647至0.969。12个位点中只有1个位点显著偏离Hardy-Weinberg平衡，未发现连锁不平衡。这些标记可用于研究红腹锦鸡儿的遗传结构。nasuspopulation.
We isolated and characterized 12 novel polymorphic microsatellite loci for the Japanese grenadier anchovy (Coilia nasus) using the method of PCR-based isolation of microsatellite arrays. Sixteen individuals ofC. nasusfrom Ariake Bay in Japan were genotyped using 24 PCR primers to characterize the polymorphism at each locus. The average number of the observed alleles per locus was 11.08 (ranging from 8 to 20). The observed heterozygosity values ranged from 0.562 to 1.0, and the expected heterozygosity values ranged from 0.647 to 0.969. Only one of the twelve loci deviated significantly from Hardy–Weinberg equilibrium, and linkage disequilibrium was not found in any pairs of the loci. These markers can be used to investigate the genetic structure of theC. nasuspopulation.